Brilliant Violet 605™ anti-human CD62P (P-Selectin) Antibody

Pricing & Availability
Clone
AK4 (See other available formats)
Workshop
VI P-44
Other Names
GMP-140, PADGEM
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
AK4_BV605_CD62P_Antibody_FC_032114.jpg
Thrombin-activated human peripheral blood platelets were stained with CD62P (clone AK4) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
  • AK4_BV605_CD62P_Antibody_FC_032114.jpg
    Thrombin-activated human peripheral blood platelets were stained with CD62P (clone AK4) Brilliant Violet 605™ (filled histogram) or mouse IgG1, κ Brilliant Violet 605™ isotype control (open histogram).
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Avail. Save
304919 25 tests $190
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304920 100 tests $380
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Description

CD62P is a 140 kD type I transmembrane glycoprotein also known as P-selectin, platelet activation-dependent granule membrane protein (PADGEM), and GMP-140. It is expressed on activated platelets, megakaryocytes, and endothelial cells. CD62P is primarily stored in secretory α-granules in platelets and Weibel-Palade bodies in endothelial cells, and is rapidly relocated to the plasma membrane upon activation. The ligands for CD62P are CD162 and CD24. A primary function of CD62P is cell adhesion during neutrophil rolling, and platelet-neutrophil and platelet-monocyte interactions.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Chimpanzee
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 605™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of acetone-fixed frozen tissue sections4 and in vitro blocking of adhesion of platelets1. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 304911).

Application References

(PubMed link indicates BioLegend citation)
  1. Skinner M, et al. 1991. J. Biol. Chem. 266:5371. (Block)
  2. Kishimoto T, et al. Eds. 1997. Leucocyte Typing VI. Garland Publishing Inc. London.
  3. Yen YT, et al. 2006. J. Virol. 80:2684.
  4. Sato Y, et al. 2005. Blood 106:428. (IHC)
Product Citations
  1. Li J, et al. 2015. Nat Commun. 6: 7737. PubMed
Publication Library
RRID
AB_2563411 (BioLegend Cat. No. 304919)
AB_2563412 (BioLegend Cat. No. 304920)

Antigen Details

Structure
Selectin, type I glycoprotein, 140 kD
Distribution

Activated platelets, megakaryocytes, endothelial cells

Function
Adhesion, neutrophil rolling, platelet-neutrophil and platelet-monocyte interactions
Ligand/Receptor
CD162 (PSGL-1), CD24 and sialylated Lewis X
Cell Type
Endothelial cells, Megakaryocytes, Neutrophils, Platelets
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. McEver R, et al. 1995. J. Biol. Chem. 270:11025.
2. Varki A. 1994. P. Natl. Acad. Sci. USA 91:7390.

Gene ID
6403 View all products for this Gene ID
UniProt
View information about CD62P on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 04/15/2014

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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