Brilliant Violet 605™ anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
C1.7 (See other available formats)
Workshop
VII 70350
Other Names
2B4, SLAMF4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C1-7_BV605_CD244_Antibody_082219
Human peripheral blood lymphocytes were stained with CD8a APC/Cyanine7 and CD244 (2B4) (clone C1.7) Brilliant Violet 605™ (left) or mouse IgG1, κ Brilliant Violet 605™ isotype control (right).
  • C1-7_BV605_CD244_Antibody_082219
    Human peripheral blood lymphocytes were stained with CD8a APC/Cyanine7 and CD244 (2B4) (clone C1.7) Brilliant Violet 605™ (left) or mouse IgG1, κ Brilliant Violet 605™ isotype control (right).
See Brilliant Violet 605™ spectral data
Cat # Size Price Quantity Avail. Save
329535 25 tests $195
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329536 100 tests $385
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Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 605™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 605™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 605™ excites at 405 nm and emits at 603 nm. The bandpass filter 610/20 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 605™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397.
  2. Chuang SS, et al. 2000. Immunology. 100:378.
  3. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed
  4. Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Lee KM, et al. 2003. J. Immunol. 170:4881.
2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
3. Messmer B, et al. 2006. J. Immunol. 176:4646.
4. Boles KS, et al. 2001. Immunol. Rev. 181:234.

Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 08/22/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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