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Streptavidin binds to biotin with high affinity. Streptavidin-Brilliant Violet 510™ is useful for detecting biotinylated antibodies. The excitation of Brilliant Violet 510™ by 405 nm laser light induces a fluorescence maximum emission of 510 nm. Streptavidin-Brilliant Violet 510™ is 340 kD.Product Details
- Human, Mouse, Rat, All Species
For Cat. No. 405234: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
For Cat No. 405233: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
- Streptavidin was conjugated with Brilliant Violet 510™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 510™.
Cat. No. 405234 is bottled at 0.5 mg/ml.
Cat. No. 405233 is bottled at 0.1 mg/ml.
- Storage & Handling
- The streptavidin solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
FC, ICFC - Quality tested
- Recommended Usage
Each lot of this Streptavidin-Brilliant Violet 510™ is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the Brilliant Violet 510™ conjugation. For immunofluorescent staining for Cat. No. 405233, we recommend using ≤0.25 µg in 100 µl staining volume per million cells. For immunofluorescent staining for Cat. No. 405234, we recommend using ≤0.1 µg in 100 µl staining volume per million cells. It is recommended that the reagent be titrated for optimal performance for each application.
Brilliant Violet 510™ excites at 405 nm and emits at 510 nm. The bandpass filter 510/50 nm is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. Brilliant Violet 510™ is a trademark of Sirigen Group Ltd.
Learn more about Brilliant Violet™.
This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
- Excitation Laser
Violet Laser (405 nm)
- Application Notes
The average molecular weight of Streptavidin-Brilliant Violet 510™ is 340 kD and Streptavidin alone is 52 kD.
(PubMed link indicates BioLegend citation)
- Mise-Omata S, et al. 2014. Int Immunol. 26:607. PubMed
- Product Citations