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Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl Antibody

Pricing & Availability
Clone
RTK2758 (See other available formats)
Regulatory Status
RUO
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
400535 125 µL $165
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400549 50 µg $248
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400536 500 µL $358
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Description

The RTK2758 immunoglobulin reacts with KLH. The isotype of this antibody is rat IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissues.

Product Details
Technical data sheet

Product Details

Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Trinitrophenol + KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The immunoglobulin was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
µg sizes: 0.2 mg/mL
µL sizes: lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis as negative control. Use at concentrations comparable to those of the specific antibody of interest.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Intracellular Flow Cytometry (ICFC), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB), and Functional Assay (FA).

Application References

(PubMed link indicates BioLegend citation)
  1. Nishimoto H, et al. 2005. Blood 106:4241.
  2. Seach N, et al. 2008. J. Immunol. 180:5384. PubMed
  3. Jiang P, et al. 1999. J. Biol. Chem. 274:559. (FA)
Product Citations
  1. Li B, Schmidt N 2016. PLoS One. 11: 0162427. PubMed
  2. Choi H et al. 2019. Cell Rep. 27(3):806-819 . PubMed
  3. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  4. Lu Y, et al. 2020. Cell. 180(6):1081-1097. PubMed
  5. Fulham MA, et al. 2019. Am J Physiol Cell Physiol. 317:C687. PubMed
  6. Gordon-Alonso M,et al. 2017. Nat. Commun. 10.1038/s41467-017-00925-6. PubMed
  7. Tan C, et al. 2020. Front Cell Dev Biol. 8:579157. PubMed
  8. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  9. Dubrot J, et al. 2021. Immunity. 54(3):571-585.e6. PubMed
RRID
AB_10933427 (BioLegend Cat. No. 400535)

Antigen Details

Gene ID
NA

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Other Formats

View All Reagents Request Custom Conjugation
Description Clone Applications
APC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Biotin Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
FITC Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA,ChIP
PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Pacific Blue™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl RTK2758 ICC
GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC,WB,IP,ICC,IHC,FA
APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl RTK2758 PG
TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl RTK2758 SB
PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl RTK2758 FC,ICFC
Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl RTK2758 FC
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • APC Rat IgG2a, κ Isotype Ctrl

  • Biotin Rat IgG2a, κ Isotype Ctrl

  • FITC Rat IgG2a, κ Isotype Ctrl

  • PE Rat IgG2a, κ Isotype Ctrl

  • PE/Cyanine5 Rat IgG2a, κ Isotype Ctrl

  • Purified Rat IgG2a, κ Isotype Ctrl

    RTK2758_GoChIP_IgG2a_Iso-Con_060618_updated.png
    Chromatin Immunoprecipitations (ChIP) were performed with cr...

  • PE/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • APC/Cyanine7 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 488 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 647 Rat IgG2a, κ Isotype Ctrl

  • Pacific Blue™ Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 700 Rat IgG2a, κ Isotype Ctrl

  • PerCP Rat IgG2a, κ Isotype Ctrl

  • PerCP/Cyanine5.5 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 421™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 570™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 605™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 650™ Rat IgG2a, κ Isotype Ctrl

  • Ultra-LEAF™ Purified Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 785™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 510™ Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 711™ Rat IgG2a, κ Isotype Ctrl

  • PE/Dazzle™ 594 Rat IgG2a, κ Isotype Ctrl

  • Alexa Fluor® 594 Rat IgG2a, κ Isotype Ctrl

  • GoInVivo™ Purified Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 750 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl

  • KIRAVIA Blue 520™ Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 550 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-C0238 Rat IgG2a, κ Isotype Ctrl

  • APC/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-B0238 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 538 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 581 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 593 Rat IgG2a, κ Isotype Ctrl

  • Spark Violet™ 423 Rat IgG2a, κ Isotype Ctrl

  • Spark NIR™ 685 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-D0238 Rat IgG2a, κ Isotype Ctrl

  • TotalSeq™-Bn0238 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 700 Rat IgG2a, κ Isotype Ctrl

  • Brilliant Violet 750™ Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 810 Rat IgG2a, κ Isotype Ctrl

  • Spark YG™ 570 Rat IgG2a, κ Isotype Ctrl

  • PE/Fire™ 640 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 574 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 806 Rat IgG2a, κ Isotype Ctrl

  • Spark Blue™ 515 Rat IgG2a, κ Isotype Ctrl

  • PerCP/Fire™ 780 Rat IgG2a, κ Isotype Ctrl

  • Spark Red™ 718 Rat IgG2a, κ Isotype Ctrl

  • Spark PLUS UV™ 395 Rat IgG2a, κ Isotype Ctrl

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