Loading...

Brilliant Violet 421™ anti-mouse CD366 (Tim-3) Antibody

Pricing & Availability
Clone
RMT3-23 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing 3 protein, hepatitis virus cellular receptor 2, CD366
Isotype
Rat IgG2a, κ
Ave. Rating
Submit a Review
Product Citations
publications
RMT3dash23_BV421_CD366_Antibody_062416
Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3, clone RMT3-23) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
  • RMT3dash23_BV421_CD366_Antibody_062416
    Mouse Tim-3 transfected cells were stained with anti-mouse CD366 (Tim-3, clone RMT3-23) Brilliant Violet 421™ (filled histogram) or rat IgG2a, κ Brilliant Violet 421™ isotype control (open histogram).
Compare all formats See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Check Availability Save
119723 50 µg $292
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

CD366 (Tim-3) is a transmembrane protein also known as T cell immunoglobulin and mucin domain containing protein-3. Tim-3 is expressed at high levels on Th1 lymphocytes and CD11b+ macrophages. Tim-3 has also been shown to exist as a soluble protein. Cells expressing Tim-3 are present at high levels in the CNS of animals at the onset of experimental autoimmune encephalomyelitis (EAE), a disease mediated by lymphocytes secreting Th1-like cytokines. Tim-3 has been proposed to inhibit Th1-mediated immune responses and promote immunological tolerance.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for relevant formats) include: in vitro1 and in vivo2 blocking of Tim-3, and immunohistochemical staining of frozen sections2. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 119731-119736).

Application References

(PubMed link indicates BioLegend citation)
  1. Nakae S, et al. 2007. Blood 110(7):2565-8. (FC, Block)
  2. Oikawa T, et al. 2006. J. Immunol. 177(7):4281-7. (FC, Block, IHC)
Product Citations
  1. Hudson WH, et al. 2020. Immunity. 51(6):1043-1058.e4.. PubMed
  2. Cribioli E, et al. 2022. Front Immunol. 13:976628. PubMed
  3. LaFleur MW, et al. 2019. Nat Commun. 10:1668. PubMed
  4. Li C, et al. 2020. Immunity. 52(1):201-202. PubMed
  5. Sun M, et al. 2021. J Immunother Cancer. 9:. PubMed
  6. Lou Y, et al. 2021. Int J Mol Sci. 22:. PubMed
  7. Raju S, et al. 2020. Cell Reports. 29(9):2556-2564.e3.. PubMed
  8. Truckenbrod EN, et al. 2021. Elife. 10:. PubMed
  9. Wang X, et al. 2021. Theranostics. 11:209. PubMed
RRID
AB_2616908 (BioLegend Cat. No. 119723)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain; predicted molecular weight 31 kD; can exist as a soluble form lacking mucin and transmembrane domains
Distribution

Expressed on activated Th1 lymphocytes and CD11b+ macrophages

Function
May play a role in the development of immune responses and the development of Th1-mediated responses
Ligand/Receptor
Putative ligand on resting CD4+ lymphocytes
Cell Type
Macrophages, Th1
Biology Area
Immunology, Inhibitory Molecules
Molecular Family
CD Molecules, Immune Checkpoint Receptors
Antigen References

1. Sabatos CA, et al. 2003. Nat. Immunol. 4:1102.
2. Sanchez-Fueyo A, et al. 2003. Nat. Immunol. 4:1102.
3. Kuchroo VK, et al. 2003. Nat. Rev. Immunol. 3:454.
4. Mooney L, et al. 2002. Nature 415:536.

Gene ID
171285 View all products for this Gene ID
UniProt
View information about CD366 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 06/24/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-mouse CD366 (Tim-3)

  • PE anti-mouse CD366 (Tim-3)

  • APC anti-mouse CD366 (Tim-3)

  • PE/Cyanine7 anti-mouse CD366 (Tim-3)

  • Biotin anti-mouse CD366 (Tim-3)

  • PerCP/Cyanine5.5 anti-mouse CD366 (Tim-3)

  • GoInVivo™ Purified anti-mouse CD366 (Tim-3)

  • Brilliant Violet 605™ anti-mouse CD366 (Tim-3)

  • Brilliant Violet 421™ anti-mouse CD366 (Tim-3)

  • Brilliant Violet 711™ anti-mouse CD366 (Tim-3)

  • Brilliant Violet 785™ anti-mouse CD366 (Tim-3)

  • TotalSeq™-A0003 anti-mouse CD366 (Tim-3)

  • Ultra-LEAF™ Purified anti-mouse CD366 (Tim-3)

  • APC/Fire™ 750 anti-mouse CD366 (Tim-3)

  • TotalSeq™-C0003 anti-mouse CD366 (Tim-3)

  • TotalSeq™-B0003 anti-mouse CD366 (Tim-3)

  • Alexa Fluor® 647 anti-mouse CD366 (Tim-3)

  • PE/Fire™ 810 anti-mouse CD366 (Tim-3)

  • PE/Dazzle™ 594 anti-mouse CD366 (Tim-3)

  • PE/Fire™ 640 anti-mouse CD366 (Tim-3)
ProductsHere
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account