Brilliant Violet 421™ anti-human IL-4 Antibody

Pricing & Availability
Clone
MP4-25D2 (See other available formats)
Other Names
Interleukin-4, Ia inducing factor (IaIF), B-cell stimulating factor-1 (BSF-1), Hodgkin's cell growth factor (HCGF), Mast cell growth factor-2 (MCGF-2), Macrophage fusion factor (MFF), T cell growth factor-2 (TCGF-2)
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
MP4-25D2_BV421_1_042211
Human peripheral blood lymphocytes were stimulated with PMA + ionomycin for 6 hours (in the presence of monensin), surface stained with CD3 FITC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • MP4-25D2_BV421_1_042211
    Human peripheral blood lymphocytes were stimulated with PMA + ionomycin for 6 hours (in the presence of monensin), surface stained with CD3 FITC, fixed, permeabilized and then stained with IL-4 (clone MP4-25D2) Brilliant Violet 421™ (top) or rat IgG1, κ Brilliant Violet 421™ isotype control (bottom).
  • MP4-25D2_BV421_2_042211
See Brilliant Violet 421™ spectral data
Cat # Size Price Quantity Avail. Save
500825 25 tests $180
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500826 100 tests $375
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Description

IL-4 is a pleiotropic cytokine that is produced by activated T cells, mast cells, and basophils. IL-4 elicits many different biological responses but has two dominant functions. The first is regulating differentiation of naïve CD4+ T cell to the Th2 type. Th2 cells produce IL-4, IL-5, IL-10, and IL-13, which tend to favor a humoral immune response while suppressing a cell-mediated immune response controlled by Th1 cells. The second is regulating IgE and IgG1 production by B cells.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Swine (Pig, Porcine), Rhesus
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
CHO-expressed, recombinant human IL-4
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions. The solution is free of unconjugated Brilliant Violet 421™ and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.


Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

ELISA Detection1,3 or ELISPOT Detection4,5: The biotinylated MP4-25D2 antibody is useful as a detection antibody for a sandwich ELISA or ELISPOT assay, when used in conjunction with purified 8D4-8 antibody (Cat. No. 500702/500707) as the capture antibody.
Flow Cytometry6,9: The fluorochrome-labeled MP4-25D2 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IL-4 -producing cells within mixed cell populations.
Neutralization1-3: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-4 bioactivity (Cat. No. 500815). The MP4-25D2 antibody can neutralize the bioactivity of natural or recombinant IL-4.

Application References

(PubMed link indicates BioLegend citation)
  1. Chretien I, et al. 1989. J. Immunol. Methods 117:67. (ELISA Detection, Neut)
  2. Ramanathan L, et al. 1993. Biochem. 32:3549. (Neut)
  3. Abrams J, et al. 1992. Immunol. Rev. 127:5. (ELISA Detection, Neut)
  4. Mahanty S, et al. 1992. J. Immunol. 148:3567. (ELISPOT Detection)
  5. Klinman D, et al. 1994. Curr. Prot. Immunol. John Wiley and Sons New York. Unit 6.19. (ELISPOT Detection)
  6. Prussin C, et al. 1995. J. Immunol. Methods 188:117. (ICFC)
  7. Raqib R, et al. 1995. Infect. Immun. 63:289.
  8. Andersson J, et al. 1994. Immunology 83:16.
  9. Iwamoto S, et al. 2007. J. Immunol. 179:1449. (ICFC) PubMed
  10. Kubota M, et al. 1997. J. Immunol. 158:5321.
  11. Dzhagalov I, et al. 2007. J. Immunol. 178:2113. PubMed
  12. Kroneke MA, et al. 2012. J. Immunol. 188:3734. PubMed
Product Citations
  1. Bal S, et al. 2016. Nat Immunol. 10.1038/ni.3444. PubMed
  2. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
Publication Library
RRID
AB_10898316 (BioLegend Cat. No. 500825)
AB_2561679 (BioLegend Cat. No. 500826)

Antigen Details

Structure
Cytokine; 15-19 kD (Mammalian)
Bioactivity
Differentiation of naïve CD4+ T cells to the TH2 type, proliferation/differentiation of activated B cells, expression of class II MHC antigens, and of low affinity IgE receptors in resting B cells
Cell Sources
Mast cells, T cells, bone marrow stromal cells
Cell Targets
B cells, T cells, monocytes, endothelial cells, fibroblasts
Receptors
Heterodimer IL-4Rα (CD124); γ-subunit (CD132) in common with IL-2R, IL-7R, IL-13R, IL-15R
Cell Type
Tregs
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Boulay J, et al. 1992. Curr. Opin. Immunol. 4:294.
3. Dullens H, et al. 1991. In vivo 5:567.
4. Paul W. 1991. Blood 77:1859.

Regulation
Upregulated by IL-2, platelet activating factor; downregulated by TGF-β
Gene ID
3565 View all products for this Gene ID
UniProt
View information about IL-4 on UniProt.org

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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