Brilliant Violet 421™ anti-human IgG Fc Antibody

Pricing & Availability
M1310G05 (See other available formats)
Regulatory Status
Rat IgG2a, κ
Ave. Rating
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Product Citations
Human peripheral blood lymphocytes were stained with CD19 APC and IgG (clone M1310G05) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • M1310G05_BV421_IgG_Fc_Antibody_FC_1_042315
    Human peripheral blood lymphocytes were stained with CD19 APC and IgG (clone M1310G05) Brilliant Violet 421™ (top) or rat IgG2a, κ Brilliant Violet 421™ isotype control (bottom).
  • M1310G05_BV421_IgG_Fc_Antibody_FC_2_042315
Compare all formats See Brilliant Violet 421™ spectral data
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410703 25 tests $204
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410704 100 tests $424
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IgG Fc is a homodimer that is composed of the constant region of the two heavy chains that form the IgG molecule. The Fc fragment mediates opsonization, antibody dependent cellular cytotoxicity (ADCC), and complement activation through binding to Fc receptors such as CD16, CD32, CD64, and the complement factor C1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Human Siglec-E-IgG Fc fusion protein.
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA).
The antibody was purified by affinity chromatography and conjugated with Brilliant Violet 421™ under optimal conditions.
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Brilliant Violet 421™ excites at 405 nm and emits at 421 nm. The standard bandpass filter 450/50 nm is recommended for detection. Brilliant Violet 421™ is a trademark of Sirigen Group Ltd.

Learn more about Brilliant Violet™.

This product is subject to proprietary rights of Sirigen Inc. and is made and sold under license from Sirigen Inc. The purchase of this product conveys to the buyer a non-transferable right to use the purchased product for research purposes only. This product may not be resold or incorporated in any manner into another product for resale. Any use for therapeutics or diagnostics is strictly prohibited. This product is covered by U.S. Patent(s), pending patent applications and foreign equivalents.
Excitation Laser
Violet Laser (405 nm)
Application Notes

Clone M1310G05 recognizes IgG in the membrane of memory B cells, has a stronger affinity for IgG1 and IgG3 than for IgG2 and IgG4, and does not cross react with IgD, IgE, or IgM.

Product Citations
  1. Guthmiller JJ, et al. 2022. Nature. 602:314. PubMed
  2. Woodruff M, et al. 2020. medRxiv. . PubMed
  3. Shuwa HA, et al. 2021. Med. 2(6):720-735.e4. PubMed
  4. Wang J, et al. 2020. Cell. 183(7):1867-1883.e26. PubMed
AB_2565625 (BioLegend Cat. No. 410703)
AB_2565626 (BioLegend Cat. No. 410704)

Antigen Details

Homodimer formed by the constant region of the IgG heavy chain.
CD16, CD32, and CD64.
Cell Type
B cells
Biology Area
Antigen References

1. Paul, WE. (2003). Fundamental Immunology. Philadelphia, PA: Lippincott, Williams, & Wilkins.

Gene ID
3500 View all products for this Gene ID
View information about IgG Fc on

Related FAQs

What is the F/P ratio range of our BV421™ format antibody reagents?

It is lot-specific. On average it ranges between 2-4.

Go To Top Version: 1    Revision Date: 04/23/2015

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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