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Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc Chimera (carrier-free)

Pricing & Availability
Regulatory Status
RUO
Other Names
B7 homolog 2 (B7H2), GL50, B7 related protein 1 (B7RP1), CD275, inducible T cell costimulator ligand ICOS Ligand), LICOS, ICOSLG
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Product Citations
publications
Human_B7-H2-_CF_RECOM_1_011823
Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera binds to immobilized human ICOS in a dose dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 2 – 10 ng/mL.
  • Human_B7-H2-_CF_RECOM_1_011823
    Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera binds to immobilized human ICOS in a dose dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding. The ED50 for this effect is 2 – 10 ng/mL.
  • Human_B7-H2-_CF_RECOM_2_011823
    Testing for Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc Chimera. Biotinylated Recombinant Human B7-H2 (ICOSL)-Fc chimera was aliquoted in PBS, 5% glycerol at 0.2 mg/mL and one aliquot was kept at 4°C (Control), and another was frozen and thawed four times (4x Freeze/Thaw). The samples were tested by their ability to bind to immobilized human ICOS in a dose dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding). The ED50 for this effect is 2 – 10 ng/mL.
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798704 25 µg $295
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798706 100 µg $495
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Description

ICOSL (B7-H2) belongs to the B7 family of immunoregulatory molecules and is a transmembrane glycoprotein of approximately 60 kD. Mature human B7-H2 consists of a 238 amino acid extracellular domain (ECD), two immunoglobulin-like domains, a 21 amino acid transmembrane segment, and a 25 residue cytoplasmic domain. Within the ECD, human B7-H2 shares 50% and 54% amino acid sequence identity with mouse and rat B7-H2, respectively. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, dendritic cells, and also in monocytes. It binds to ICOS and is expressed on activated T cells, which leads to both positive and negative effects on immune responses including its own downregulation. Mouse and human B7-H2 exhibit cross species binding to ICOS. B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells, and germinal centers. In addition, human B7-H2 binds to CD28 and CTLA4.  The interaction of human B7-H2 and CD28 can costimulate both human and mouse naïve T cells and regulatory T cells (Treg). B7-H2 plays a role in the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lungs. B7-H2 activation of ICOS on Tregs limits pulmonary inflammation and airway hyper-responsiveness, promotes the development of inhalational tolerance, and impairs anti-tumor immunity.

Product Details
Technical data sheet

Product Details

Source
Human B7-H2, amino acids (Asp19-Ser258) (Accession# O75144.2), with a linker (GS), a C-terminal human IgG1 (Pro100-Lys330) and an Avi-tag, was expressed in CHO cells. Human ICOSL-Avi tag was site-specifically biotinylated by enzyme BirA.
Molecular Mass
The 488 amino acid recombinant protein has a predicted molecular mass of approximately 54.58 kD. The DTT-reduced and non-reduced protein migrates at approximately 80 kD and 150 kD by SDS-PAGE, respectively. The predicted N-terminal amino acid is Asp.
Purity
> 95%, as determined by Coomassie stained SDS-PAGE.
Formulation
0.22 µm filtered protein solution is in PBS, 5% glycerol.
Endotoxin Level
Less than 0.1 EU per µg cytokine as determined by the LAL method.
Concentration
25 µg size is bottled at 200 µg/mL. 100 µg size and larger sizes are lot-specific and bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Unopened vial can be stored between 2°C and 8°C for up to 2 weeks, at -20°C for up to six months, or at -70°C or colder until the expiration date. For maximum results, quick spin vial prior to opening. The protein can be aliquoted and stored at -20°C or colder. Stock solutions can also be prepared at 50 – 100 µg/mL in appropriate sterile buffer, carrier protein such as 0.2 – 1% BSA or HSA can be added when preparing the stock solution. Aliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
Activity
Biotinylated recombinant human B7-H2 (ICOSL)-Fc binds to immobilized human ICOS in a dose dependent manner. HRP Avidin (Cat. No. 405103) was used to detect the binding). The ED50 for this effect is 2 – 10 ng/mL.
Application

Bioassay
Application Notes

BioLegend carrier-free recombinant proteins provided in liquid format are shipped on blue-ice. Our comparison testing data indicates that when handled and stored as recommended, the liquid format has equal or better stability and shelf-life compared to commercially available lyophilized proteins after reconstitution. Our liquid proteins are validated in-house to maintain activity after shipping on blue ice and are backed by our 100% satisfaction guarantee. If you have any concerns, contact us at tech@biolegend.com.

Antigen Details

Structure
Homodimer
Distribution

B cells, macrophages, monocytes, and dendritic cells

Function
The B7-H2/ICOS signal transduction induces T cell proliferation and secretion of both Th1 and Th2 cytokines, such as IL-4 and IFN-γ, but not IL-2 ( it also induces IL-5 and TNF-α production). B7-H2/ICOS signal may favor the induction of Th2-type responses and thus may be involved in the negative regulation of cell-mediated immune responses against tumors. B7-H2 is induced by TNF-α in B cells and monocytes and downregulated in dendritic cells.
Ligand/Receptor
ICOS (CD278)
Bioactivity
Measured by its ability to bind recombinant human ICOS
Cell Type
Dendritic cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Costimulatory Molecules, Immunology, Signal Transduction
Molecular Family
Soluble Receptors, TCRs
Antigen References
  1. Ishikawa K, et al. 1998. DNA Res. 5:169-76.
  2. Yoshinaga SK, et al. 2000. Int. Immunol. 12:1439-47.
  3. Flesch IE, 2003. J Biol Regul Homeost Agents. 16:217-9.
  4. Akbari O, et al. 2002. Nat. Med. 8:1024-32.
  5. Yao S, et al. 2011. Immunity. 34:29-40.
Gene ID
23308 View all products for this Gene ID
UniProt
View information about B7-H2 on UniProt.org
Go To Top Version: 1    Revision Date: 01/18/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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