Biotin anti-Tau, 189-195 Antibody

Pricing & Availability
Clone
39E10 (See other available formats)
Other Names
Microtubule-associated protein tau, PHF-tau, Paired Helical Filament tau
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
39E10_Biotin_Tau_189-195_Antibody_1_WB_010517
Western blot of biotin anti-Tau, 189-195 antibody (clone 39E10) and isotype-matched control IgG1. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blots were treated with biotin block, incubated for two hours at room temperature with 5 µg/mL of the primary antibody or mouse IgG1, followed by incubation with Streptavidin-HRP (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • 39E10_Biotin_Tau_189-195_Antibody_1_WB_010517
    Western blot of biotin anti-Tau, 189-195 antibody (clone 39E10) and isotype-matched control IgG1. Lane 1: 20 µg of human brain lysate; Lane 2: 20 µg of rat brain lysate; Lane 3: 20 µg of mouse brain lysate. The blots were treated with biotin block, incubated for two hours at room temperature with 5 µg/mL of the primary antibody or mouse IgG1, followed by incubation with Streptavidin-HRP (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • 39E10_Biotin_Tau_189-195_Antibody_2_ELISA_010917
    Direct ELISA of biotin anti-Tau, 189-195 antibody (clone 39E10) binding to plate-immobilized recombinant human 2N3R and 2N4R tau proteins. ELISA was performed by coating wells with 150 ng of each recombinant tau protein for two hours at 37°C. The wells were then incubated with the primary antibody at 37°C for one hour, followed by incubation with horseradish peroxidase labeled HRP goat anti-mouse IgG antibody. TMB (3, 3', 5, 5' tetramethylbenzidine) was used as the detection system
Cat # Size Price Quantity Avail. Save
814303 25 µg $105
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814304 100 µg $255
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Description

Tau protein promotes microtubule assembly and stability. Tau is abundant in neurons of the central nervous system, and is expressed at low levels in astrocytes and oligodendrocytes. Abnormal hyper-phosphorylation, aggregation, and toxic gain of function of tau is associated with several neurological disorders, including Alzheimer’s disease (AD). The major building block of neurofibrillary lesions in AD brains consists of paired helical filaments (PHFs) of abnormally hyperphosphorylated tau. Six isoforms of tau are generated by alternative splicing of the MAPT gene. These isoforms are distinguished by the number of tubulin binding domains, 3 (3R) or 4 (4R), in the C-terminal of the protein and by one (1N), two (2N), or no (0N) inserts in the N-terminal domain.  Tau isoforms are differentially expressed during development.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against full-length human tau protein.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
Direct ELISA - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 µg per ml.  For Direct ELISA, the suggested use of this reagent is 10 ng to 1.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB).

BioLegend is the Exclusive Provider of this Antibody Product

Application References

(PubMed link indicates BioLegend citation)
  1. Michalski V, et.al. Neurobiol Aging. 2015. 36(12):3131. (WB) PubMed
RRID
AB_2650699 (BioLegend Cat. No. 814303)
AB_2650700 (BioLegend Cat. No. 814304)

Antigen Details

Structure
Unmodified Tau isoforms have an apparent molecular weight ranging from 33-79 kD. Additional high and low molecular weight Tau species have been observed in brain tissues.
Distribution

Tissue distribution: Central nervous system, peripheral ganglia and nerves, kidney, skeletal, and heart muscle.
Cellular distribution: Cytoskeleton, nucleus, plasma membrane, and cytosol.

Function
Tau promotes microtubule assembly and stability. The short tau isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.
Interaction
Tau interacts with: Sequestosome-1, Peptidyl-prolyl cis-trans isomerase FKBP4, Casein kinase I isoform delta, Serine/threonine-protein kinase Sgk1, Laforin, and alpha-synuclein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
Tau
Antigen References

1. Meredith JE Jr, et al. 2013. PLoS One. 8(10): e76523. PubMed
2. Goodall CA, et al. 2006. J. Neurol. Neurosurg. Psychiatry 77(1): 89. PubMed
3. Wang Y, Mandelkow E. 2016. Nat. Rev. Neurosci. 17(1):5-21. PubMed

Gene ID
4137 View all products for this Gene ID
UniProt
View information about Tau on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 01/29/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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