Biotin anti-Synaptotagmin-12 Antibody

Pricing & Availability
Clone
N277/7 (See other available formats)
Other Names
Synaptotagmin-12, sytXII, synaptotagmin 12, Syt12, Synaptotagmin XII, Synaptotagmin-related gene 1 protein, Srg1, Sytr1
Isotype
Mouse IgG1
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Product Citations
publications
N2777_Biotin_Synaptotagmin12_Antibody_1_090618
Western blot of Biotin anti-Synaptotagmin-12 antibody (clone N277/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 5 µg of mouse brain membrane lysate; Lane 4: 5 µg of rat brain membrane lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • N2777_Biotin_Synaptotagmin12_Antibody_1_090618
    Western blot of Biotin anti-Synaptotagmin-12 antibody (clone N277/7). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain membrane lysate; Lane 3: 5 µg of mouse brain membrane lysate; Lane 4: 5 µg of rat brain membrane lysate. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • N2777_Biotin_Synaptotagmin12_Antibody_2_090618
    IHC staining of Biotin anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slide was counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N2777_Biotin_Synaptotagmin12_Antibody_3_090618
    IHC staining of Biotin anti-Synaptotagmin-12 antibody (clone N277/7) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). The slide was counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
820407 25 µg $90
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820408 100 µg $160
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Description

Synaptotagmin 12 (Syt12) is regulated by protein kinase A (PKA) and is unique from other synaptotagmins in that it does not bind to Ca2+. Syt12 interaction with Syt1 prevents SNARE complex binding. Expression of Syt12 induces spontaneous neurotransmitter release, providing a mechanism for spontaneous synaptic-vesicle exocytosis that is Ca2+-independent.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 168-255 (cytoplasmic C2A domain) of mouse Synaptotagmin-12.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC-P).

RRID
AB_2750400 (BioLegend Cat. No. 820407)
AB_2750401 (BioLegend Cat. No. 820408)

Antigen Details

Structure
Expected MW: 47 kD
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Presynaptic proteins
Gene ID
171180 View all products for this Gene ID
UniProt
View information about Synaptotagmin-12 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 09/06/2018

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