- 1A29 (See other available formats)
- Other Names
- Mouse IgG1, κ
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CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.Product Details
- Antibody Type
- Host Species
- HEV-derived cell line Ax
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.
(PubMed link indicates BioLegend citation)
- Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
- Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
- Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
- Willenborg DO, et al. 1996. J. Immunol. 157:1973.
- McKechnie NM, et al. 2002. IVOS 43:411. (IHC)
AB_2121900 (BioLegend Cat. No. 202403)
- Ig superfamily, 85 kD
High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.
- Cellular adhesion, inflammation, immune reactions
- CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, hyaluronan
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Granulocytes, Monocytes, T cells
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
1. Tamatani T, et al. 1990. Int. Immunol. 2:165.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.
- Gene ID
- 15894 View all products for this Gene ID
- View information about CD54 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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