Biotin anti-rat CD54 Antibody

Pricing & Availability
1A29 (See other available formats)
Regulatory Status
Other Names
Mouse IgG1, κ
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Product Citations
LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE
  • 1A29_biotin_030410
    LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE
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202403 100 µg $143
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CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
HEV-derived cell line Ax
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
  2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
  3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
  4. Willenborg DO, et al. 1996. J. Immunol. 157:1973.
  5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)
AB_2121900 (BioLegend Cat. No. 202403)

Antigen Details

Ig superfamily, 85 kD

High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.

Cellular adhesion, inflammation, immune reactions
CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, hyaluronan
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tamatani T, et al. 1990. Int. Immunol. 2:165.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.

Gene ID
15894 View all products for this Gene ID
View information about CD54 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-rat CD54

  • PE anti-rat CD54


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