Biotin anti-rat CD54 Antibody

Pricing & Availability
Clone
1A29 (See other available formats)
Other Names
ICAM-1
Isotype
Mouse IgG1, κ
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Product Citations
publications
1A29_biotin_030410
LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE
  • 1A29_biotin_030410
    LOU rat splenocytes stained with biotinylated 1A29, followed by SAV-PE
Cat # Size Price Quantity Avail. Save
202403 100 µg $130
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Description

CD54 is an 85 kD protein also known as ICAM-1. It is a member of the immunoglobulin superfamily that is expressed on high endothelial venules in lymphoid tissue, peripheral blood monocytes, dendritic cells, granulocytes and thymic stromal cells. CD54 is weakly expressed on T and B cells, although expression can be increased by activation (especially on endothelial cells). ICAM-1 is an important protein in cell adhesion, inflammation and immune responses. CD54 has been shown to bind to CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, and hyaluronan. The 1A29 antibody has been reported to block T cell aggregation and binding of lymphocytes to HEV-derived endothelial cells and purified ICAM-1 protein.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
HEV-derived cell line Ax
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemistry1,5 of acetone-fixed frozen sections and STUF-treated paraffin-embedded sections, immunoprecipitation2, in vitro and in vivo blocking of PMA-induced T cell blast aggregation and adhesion to HEV-derived endothelial cells and purified ICAM-1 protein2,3,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Clark WM, et al. 1995. Mol. Chem. Neuropathol. 26:213. (IHC)
  2. Tamatani T, et al. 1990. Int. Immunol. 2:165. (IP)
  3. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
  4. Willenborg DO, et al. 1996. J. Immunol. 157:1973.
  5. McKechnie NM, et al. 2002. IVOS 43:411. (IHC)
RRID
AB_2121900 (BioLegend Cat. No. 202403)

Antigen Details

Structure
Ig superfamily, 85 kD
Distribution

High endothelial venules in lymphoid tissues, peripheral blood monocytes, dendritic cells, granulocytes, thymic stromal cells and weakly on T and B cells. Upregulated on activated cells and endothelial cells.

Function
Cellular adhesion, inflammation, immune reactions
Ligand/Receptor
CD11a/CD18 (LFA-1), CD11b/CD18, CD11c/CD18, CD43, fibrinogen, hyaluronan
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Monocytes, T cells
Biology Area
Cell Adhesion, Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Costimulatory Molecules
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Tamatani T, et al. 1990. Int. Immunol. 2:165.
2. Tamatani T, et al. 1991. Eur. J. Immunol. 21:627.
3. Turunen JP, et al. 1993. Scand. J. Immunol. 37:282.

Gene ID
15894 View all products for this Gene ID
UniProt
View information about CD54 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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