Biotin anti-Rat Blood-Brain Barrier Antibody

Pricing & Availability
Clone
SMI 71 (See other available formats)
Other Names
Endothelial barrier antigen, EBA
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
SMI71_Biotin_Blood-Brain-Barrier_Antibody_1_022218
IHC staining of Biotin anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component No. 5, DAB; Cat. No. 929901). The tissue was counterstained with hematoxylin and bluing solution. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI71_Biotin_Blood-Brain-Barrier_Antibody_1_022218
    IHC staining of Biotin anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component No. 5, DAB; Cat. No. 929901). The tissue was counterstained with hematoxylin and bluing solution. The image was captured with a 40X objective. Scale bar: 50 µm
  • SMI71_Biotin_Blood-Brain-Barrier_Antibody_2_022218
    IHC staining of Biotin anti-Rat Blood-Brain Barrier antibody (clone SMI 71) on formalin-fixed paraffin-embedded rat brain tissue. The tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component No. 5, DAB; Cat. No. 929901). The tissue was counterstained with hematoxylin and bluing solution. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Avail. Save
836805 25 µg $115
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836806 100 µg $255
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Description

SMI 71 is specific for a rat endothelial protein found in areas with blood-brain or blood-nerve barriers. The antibody does not react with endothelia of periventricular organs or with fenestrated endothelia in peripheral tissues. Specifically, it has been shown that staining with SMI 71 is not observed in blood vessels and sinusoids in the liver, vessels in the heart, adrenal, skeletal muscle, intestine, thymus, lymph nodes, pancreas, thyroid, or skin. However, a patchy reaction was observed on some vessel walls of the spleen and epidermis of the skin3. Reactivity with the antibody develops in newborn rats along with maturation of the blood brain barrier. Reactivity disappears in lesions of experimental allergic encephalomyelitis. Contrary to the belief that astrocytes and perhaps neurons are essential for the establishment of the blood-brain barrier, destruction of neurons (monitored by SMI 311 and anti-MAP2) and of astrocytes (monitored by anti-GFAP and anti-S100B) leads only to transient abolition of SMI 71 reactivity and only transient transendothelial passage of serum albumin.

Product Details
Technical data sheet

Product Details

Reactivity
Rat
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

IHC-P - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.5 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Ghabriel MN, et al. 2002. Reproduction 123:389-97. (IHC)
  2. Beaumont M, et. al. 2009. J. Cereb. Blood Flow Metab. 29:1714. (IHC) PubMed
  3. Sternberger NH, Sternberger LA. 1987. Proc. Natl. Acad. Sci. USA 84(22):8169. (IHC) PubMed
RRID
AB_2728550 (BioLegend Cat. No. 836805)
AB_2728551 (BioLegend Cat. No. 836806)

Antigen Details

Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers
Gene ID
NA
UniProt
View information about Blood-Brain Barrier on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 0    Revision Date: 02/23/2018

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