Biotin anti-Rab7A Antibody

Pricing & Availability
Clone
W16034A (See other available formats)
Other Names
Rab7, Ras-Related Protein Rab-7a, Ras-Associated Protein RAB7
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
W16034A_Biotin_Rab7A_Antibody_122117
Western blot of Biotin anti-Rab7A antibody (clone W16034A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60-minutes at room temperature. Enhanced chemiluminescence was used as the detection system.
  • W16034A_Biotin_Rab7A_Antibody_122117
    Western blot of Biotin anti-Rab7A antibody (clone W16034A). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 5.0 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210) for 60-minutes at room temperature. Enhanced chemiluminescence was used as the detection system.
Cat # Size Price Quantity Avail. Save
850403 25 µg $125
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850404 100 µg $285
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Description

Rab7, a small GTPase of the Rab family, plays multiple roles including vesicular transport, endocytic trafficking, and autophagy. Rab7 is associated with both the endosome and lysosome, and it facilitates endosomal maturation, transport from the late endosome to the lysosome, and positioning of the endosome and lysosome via regulating their movement along cytoskeleton. Consistently, mutations or dysfunctions of Rab7 result in traffic disorders, which cause various diseases, such as neuropathy, cancer and lipid metabolism disease. Rab7 also plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Rat, Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Full-length recombinant human Rab7A protein expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2721507 (BioLegend Cat. No. 850403)
AB_2721506 (BioLegend Cat. No. 850404)

Antigen Details

Structure
Rab7A is a 207 amino acid protein with a molecular mass of 23 kD.
Distribution

Tissue distribution: Rab7A is ubiquitously expressed.
Cellular distribution: Plasma membrane, cytosol, lysosome, endosome, and Golgi apparatus.

Function
Rab7A is involved in the endocytic pathway and autophagy.
Interaction
Rab7A interacts with RILP, Rab1A, Rab11A, and UBC.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Neuroscience Cell Markers, Protein Trafficking and Clearance
Molecular Family
Autophagosome Markers
Antigen References

1. Guerra F, Bucci C. 2016. Cells. 5(3).

Gene ID
7879 View all products for this Gene ID
UniProt
View information about Rab7A on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 12/28/2017

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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