- Poly5160 (See other available formats)
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF-2
- Goat Polyclonal IgG
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- Product Citations
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TNF-α is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-α. Monomeric mouse TNF-α is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.Product Details
- Mouse, Cross-Reactivity: Rat
- Antibody Type
- Host Species
- Recombinant mouse TNF-α
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
ELISA Detection - Quality tested
- Recommended Usage
Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25 - 1.0 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of TNF-α recombinant protein ranging from 500 to 7.8 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
ELISA Detection: The biotinylated Poly5160 antibody is useful as a detection antibody for a sandwich ELISA when used in conjunction with purified TN3-19.12 antibody (Cat. Nos. 506102 & 506106) as the capture antibody for detecting mouse and rat TNF-α. The biotinylated Poly5160 antibody can also be used as a detection antibody in a sandwich ELISA to detect mouse TNF- α when used in conjunction with purified MP6-XT22 (Cat. Nos. 506301 & 506302) and recombinant mouse TNF- α (Cat. No. 575209) as the standard.
Note: For testing mouse TNF-α in serum, plasma or supernatant, BioLegend's LEGEND MAX™ Kits (Cat. Nos. 430907 & 430908) are specially developed and recommended.
- Product Citations
AB_2561411 (BioLegend Cat. No. 516003)
- TNF superfamily; dimer/trimer; 18-150 kD (Mammalian).
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells.
- Type II integral membrane protein processed by TACE for secretion; upregulated by interferons, IL-2, GM-CSF, substance P, bradykinin, PAF, immune complexes, cyclooxygenase; downregulated by IL-6, TGF-β, vitamin D3, prostaglandin E2, PAF antagonists.
- Monocytes, neutrophils, macrophages, T cells, fibroblasts, endothelial cells, osteoclasts, adipocytes, astroglia, microglia.
- TNFRSF1A (TNF-R1, CD120a, TNFR-p60 Type β, p55); TNFRSF1B (TNF-R2, CD120b, TNFR-p80 Type A, p75).
- Cell Type
- B cells, Macrophages, Monocytes, Neutrophils, NK cells, T cells
- Biology Area
- Molecular Family
- Antigen References
1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Beutler B, et al. 1988. Annu. Rev. Biochem. 57:505.
3. Beutler B, et al. 1989. Annu. Rev. Immunol. 7:625.
4. Tracey K, et al. 1993. Crit. Care Med. 21:S415.
- Gene ID
- 21926 View all products for this Gene ID
- View information about TNF-alpha on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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