- RB6-8C5 (See other available formats)
- Other Names
- Rat IgG2b, κ
- Ave. Rating
- 1 reviews
- Product Citations
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Gr-1 is a 21-25 kD protein also known as Ly-6G/Ly-6C. This myeloid differentiation antigen is a glycosylphosphatidylinositol (GPI)-linked protein expressed on granulocytes and macrophages. In bone marrow, the expression levels of Gr-1 directly correlate with granulocyte differentiation and maturation; Gr-1 is also transiently expressed on bone marrow cells in the monocyte lineage. Immature Myeloid Gr-1+ cells play a role in the development of antitumor immunity.Product Details
- Antibody Type
- Host Species
- Raised against granulocytes of mouse origin
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
FC - Quality tested
IP, IHC, WB - Reported in the literature
- Recommended Usage
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C19. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A819. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.420.
The RB6-8C5 antibody has been used to identify peripheral blood neutrophils and deplete granulocytes in vivo. Additional reported applications (for relevant formats of this clone) include: in vitro complement-mediated cytotoxicity2, in vivo depletion3-5,9, immunoprecipitation1, immunohistochemical staining6 (including paraffin-embedded sections9,16, acetone-fixed frozen sections11 and zinc-fixed sections15), and Western blotting7. RB6-8C5 is not suitable for depletion of hepatic myeloid derived suppressor cells (MDSCs)20.
Special Note: The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 108414). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 108436) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).
(PubMed link indicates BioLegend citation)
- Fleming TJ, et al. 1993. J. Immunol. 151:2399. (IP)
- Brummer E, et al. 1984. J. Leukocyte Biol. 36:505. (CMCD)
- Stoppacciaro A, et al. 1993. J. Exp. Med. 178:151. (Deplete)
- Tumpey TM, et al. 1996. J. Virol. 70:898. (Deplete)
- Czuprynski CJ, et al. 1994. J. Immunol. 152:1836. (Deplete)
- Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
- Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819. (WB)
- Engwerda CR, et al. 2004. Am. J. Pathol. 165:2123.
- Brown CR, et al. 2004. Infect. Immun. 72:4956. (Deplete, IHC)
- Andoniou CE, et al. 2005. Nature Immunology 6:1011. (FC) PubMed
- Li M, et al. 2006. P. Natl. Acad. Sci USA 103:11736. (IHC)
- Dzhagalov I, et al. 2007. Blood 109:1620. (FC) PubMed
- Fazilleau N, et al. 2007. Nature Immunol. 8:753. (FC) PubMed
- Heuser M, et al. 2007. Blood 110:1639. (FC) PubMed
- Wang T, et al. 2007. Infect. Immun. 75:1144. (IHC)
- Bosio CM, et al. 2007. J. Immunol. 178:4538. (IHC)
- Boehme SA, et al. 2009. Int. Immunol. 21:81. (IHC)
- Piao Y, et al. 2012. Neuro Oncol. 14:1379. PubMed
- Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
- Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
- Li J, et al. 2012. Arthritis Rheum. 64:1098. PubMed
- Fan Q, et al. 2014. Cancer Res. 74:471. PubMed
- Korrer MJ, et al. 2014. PLoS One. 9:91370. PubMed
- Morshed M, et al. 2014. J Immunol. 192:5314. PubMed
- Collins C, et al. 2014. PNAS. 111:9899. PubMed
- Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
- Bianchi G, et al. 2014. Cell Death Dis. 5:1135. PubMed
- Guo H, et al. 2014. J Leukoc Biol. 96:419. PubMed
- Roderick JE, et al. 2014. PNAS. 111:14436. PubMed
- Distel E, et al. 2014. Circ Res. 115:759. PubMed
- Iwai H, et al. 2015. Tuberculosis. 95:246. PubMed
- Charmsaz S, et al. 2015. PLoS One. 10:130692. PubMed
- Product Citations
AB_313368 (BioLegend Cat. No. 108403)
AB_313369 (BioLegend Cat. No. 108404)
- 21-25 kD
- Cell Type
- Granulocytes, Monocytes, Neutrophils
- Biology Area
- Immunology, Innate Immunity
- Antigen References
1. Fleming TJ, et al. 1993. J. Immunol. 151:2399.
2. Jutila MA, et al. 1988. Eur. J. Immunol. 18:1819.
3. Goni O, et al. 2002. Int. Immunol. 14:1125.
- Gene ID
- 17067 View all products for this Gene ID 546644 View all products for this Gene ID
- View information about Ly-6G/Ly-6C on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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