Biotin anti-mouse IL-33 Antibody

Pricing & Availability
Poly5165 (See other available formats)
Regulatory Status
Other Names
Il-1f11, Nuclear factor from high endothelial venules (NF-HEV)
Goat Polyclonal Ig
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Product Citations
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516503 50 µg $248
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IL-33 belongs to the IL-1 family and is closely related in structure to IL-18 and IL-1b. IL-33, IL-1b, and IL-18 are synthesized as biologically inactive precursor and are cleaved by the enzyme caspase-1 to be secreted as active mature forms. IL-33 stimulates target cells by binding to the IL-1R/TLR superfamily member ST2 and subsequently activates NF-κB and MAPK pathways via identical signalling events to those observed for IL-1b. In addition, IL-33 is a nuclear factor (NF-HEV) abundantly expressed in high endothelial venules from lymphoid organs that associates with chromatin and exhibits transcriptional regulatory properties.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Host Species
Mouse IL-33, amino acids Ser109-Ile266 (Accession# NM_133775) was expressed in E. coli.
Phosphate-buffered solution, pH 7.2, containing 1% BSA (USA origin) and 0.09% sodium azide.
The antibody was purified by antigen affinity chromatography, and conjugated with biotin under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.25-1 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance. To obtain a linear standard curve, serial dilutions of IL-33 recombinant protein ranging from 1000 to 15.6 pg/ml are recommended.

Application Notes

ELISA Detection: The biotinylated Poly5165 antibody is useful as a detection antibody in a sandwich ELISA assay, when used in conjunction with purified Poly5165 (Cat. No. 516501) antibody as the capture antibody and recombinant mouse IL-33 (Cat. No. 580509) as the standard.

Application References

(PubMed link indicates BioLegend citation)
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed
Product Citations
  1. Gao X, et al. 2015. J Immunol. 194:438. PubMed
AB_2561415 (BioLegend Cat. No. 516503)

Antigen Details


High levels of mouse IL-33 mRNA are detected in the stomach, lung, spinal cord, brain, and skin. Expression within these organs is restricted to a few cell types, such as epithelial cells, fibroblasts, and smooth muscle cells. In addition, mouse IL-33 mRNA is found in resting dendritic cells and activated macrophages. Endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues.

IL-33 drives production of Th2-associated cytokines from in vitro polarized Th2 cells. In mice, IL-33 injection induced the expression of IL-4, IL-5, and IL-13 and led to severe pathological changes in the lung and the digestive tract. In addition, IL-33 acts as a chemoattractant for Th2 cells, both in vitro and in vivo. TNF-a and IL-1b are activators of IL-33 transcription in fibroblasts and keratinocytes.
IL-33 binds to the IL-1 family receptor T1/ST2 and IL-1RAcP (IL-1 receptor associated protein).
Cell Type
Epithelial cells, Fibroblasts
Biology Area
Molecular Family
Antigen References

1. Schmitz J, et al. Immunity 2005 23:479
2. Barksby HE, et al. C Exp Immunol 2007 149:217
3. Arend WP, et al. ImmRev 2008 223:20
4. Suzukawa M, et al. J. Immunol. 2008 181:5981
5. Moussio C, et al. PlosOne 2008 10:e3331.

Gene ID
77125 View all products for this Gene ID
View information about IL-33 on

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.

Other Formats

View All IL-33 Reagents Request Custom Conjugation
Description Clone Applications
Biotin anti-mouse IL-33 Poly5165 ELISA Detection
Purified anti-mouse IL-33 Poly5165 ELISA Capture
Go To Top Version: 3    Revision Date: 12/16/2021

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Biotin anti-mouse IL-33

  • Purified anti-mouse IL-33


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