Biotin anti-mouse IL-17A Antibody

Pricing & Availability
Clone
TC11-8H4 (See other available formats)
Other Names
Interleukin-17, Cytotoxic T lymphocyte-associated antigen 8 (CTLA-8)
Isotype
Rat IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
507001 50 µg $100
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507002 500 µg $275
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Description

IL-17, also known as CTLA-8, is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpes virus Saimiri. Recent study has shown that IL-17 is produced by Th cells (Th17) that are distinct from the traditional Th1- and Th2-cell subsets. IL-23 plays an important role in triggering IL-17 production. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; activation of NF-κB and costimulation of T cell proliferation. IL-17 is an essential inflammatory mediator in the development of autoimmune diseases. Neutralization of IL-17 with monoclonal antibody is able to ameliorate the disease course. The TC11-8H4 antibody antibody reacts with mouse interleukin-17 (IL-17).

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli-expressed, recombinant mouse IL-17A
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For use as an ELISA detection antibody, a concentration range of 0.5-2 µg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-17 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes


ELISA3,4 and ELISPOT5 Detection: The biotinylated TC11-8H4 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified TC11-18H10 antibody (Cat. No. 506901) as the capture antibody, and with recombinant mouse IL-17 (Cat. No. 564101) as the standard.
Additional reported applications (for the relevant formats) include: Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Kennedy J, et al. 1996. J. Interferon Cytokine Res. 16:611.
  2. Schwarzenberger P, et al. 1998. J. Immunol. 161:6383.
  3. Infante-Duarte C, et al. 2000. J. Immunol. 165:6107.
  4. Harrington LE, et al. 2005. Nature Immunol. doi:10.1038/ni1254.
  5. Nekrasova T, et al. 2005. J. Immunol. 175:2734.
  6. Yu H, et al. 2010. Infect immun. 78:2272. PubMed
  7. Sosa RA, et al. 2013. J. Immunol. 191:5484. PubMed
  8. Haas KM, et al. 2014. J Infect Dis. 209:87. PubMed
  9. Honda-Okubo Y, et al. 2015. J Virol. 89:2995. PubMed
Product Citations
  1. Yu H, et al. 2010. Infect Immun. 78:2272. PubMed
  2. Sosa R, et al. 2013. J Immunol. 191:5848. PubMed
  3. Haas K, et al. 2014. J Infect Dis. 209:87. PubMed
  4. Honda-Okubo Y, et al. 2015. J Virol. 89:2995. PubMed
  5. Ji N, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: e139. PubMed
  6. Sosa R, et al. 2015. Proc Natl Acad Sci U S A. 112: 5038-5047. PubMed
Publication Library
RRID
AB_315465 (BioLegend Cat. No. 507001)
AB_315466 (BioLegend Cat. No. 507002)

Antigen Details

Structure
Cytokine; dimer; 15 kD (Mammalian)
Bioactivity
Secretion of IL-6, IL-8, G-CSF, prostaglandin E2 by epithelial, endothelial or fibroblastic cells; stimulates cell migration, cord formation, and IL-6 secretion by stromal cells
Cell Sources
CD4+ memory T cells
Cell Targets
Fibroblasts, epithelial and endothelial cells, stromal cells
Receptors
IL-17R (CD217)
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Numasaki M, et al. 2002. Blood 101:2620.
3. Fossiez F, et al. 1996. J. Exp. Med. 183:2593.
4. Yao Z, et al. 1997. Cytokine 9:794.
5. Dong C. 2006 Nat. Rev. Immunol. 6(4):329.
6. Hofstetter HH, et al. 2005 Cell. Immunol. 237(2):123.

Gene ID
16171 View all products for this Gene ID
UniProt
View information about IL-17A on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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