Biotin anti-mouse I-Ad Antibody

Pricing & Availability
Clone
39-10-8 (See other available formats)
Other Names
MHC class II
Isotype
Mouse (C3H.SW) IgG3, κ
Ave. Rating
Submit a Review
Product Citations
publications
39-10-8_Biotin_I-A_Antibody_091117
BALB/c mouse splenocytes were stained with biotinylated anti-mouse I-Ad (clone 39-10-8, filled histogram) or biotinylated mouse IgG3, κ isotype control (open histogram), followed by streptavidin PE.
  • 39-10-8_Biotin_I-A_Antibody_091117
    BALB/c mouse splenocytes were stained with biotinylated anti-mouse I-Ad (clone 39-10-8, filled histogram) or biotinylated mouse IgG3, κ isotype control (open histogram), followed by streptavidin PE.
Cat # Size Price Quantity Avail. Save
115003 50 µg $70
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

The 39-10-8 antibody reacts with the I-Ad MHC class II alloantigen. These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2d bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins. The 39-10-8 antibody does not cross-react with other haplotypes (a, b, k, p, q, s), but has been demonstrated to cross-react with the g7 haplotype.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse d and g7 haplotypes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
(C3H x BALB/c)F1 mouse cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy2, and immunohistochemical staining of acetone-fixed frozen sections. Does not cross-react with other haplotypes (e.g., a, b, k, p, q, s).

Application References

(PubMed link indicates BioLegend citation)
  1. Hiramine C, et al. 1995. Cell. Immunol. 160:157.
  2. Wang Z, et al. 2004. J. Immunol. 172:5924.
  3. Ma XT, et al. 2006. Cancer Research 66:1169.
  4. Norian LA and Allen PM. 2004. J. Immunol. 173:835. PubMed
  5. Tian C, et al. 2007. J. Immunol. 179:6762.
Product Citations
  1. Allen L 2004. J Immunol. 173:835. PubMed
Publication Library
RRID
AB_313618 (BioLegend Cat. No. 115003)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of H-2d mice

Function
Antigen presentation
Ligand Receptor
CD3/TCR, CD4
Cell Type
B cells, T cells, Antigen-presenting cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Wall KA, et al. 1983. J. Immunol. 131:1056.
4. Ridgway WM, et al. 1998. J. Exp. Med. 188:2267.

Gene ID
14961 View all products for this Gene ID
UniProt
View information about I-Ad on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 09/11/2017

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account