- Clone
- 2F1/KLRG1 (See other available formats)
- Other Names
- MAFA, 2F1-Ag
- Isotype
- Syrian hamster IgG
- Ave. Rating
- Submit a Review
- Product Citations
- publications
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C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and biotinylated 2F1/KLRG1, followed by Sav-PE -
C57BL/6 mouse splenocytes stained with NK-1.1 (PK136) APC and biotinylated Syrian hamster IgG, followed by Sav-PE
| Cat # | Size | Price | Quantity Avail. | Save | ||
|---|---|---|---|---|---|---|
| 138405 | 50 µg | $60 | ||||
| 138406 | 500 µg | $245 | ||||
Killer cell lectin-like receptor G1 (KLRG1) is the mouse homolog of the rat mast cell function-associated antigen (MAFA or 2F1-Ag). KLRG1 is a type II membrane glycoprotein that was first identified on the surface of rat mast cell line RBL-2H3. It is composed of a homodimer of glycosylated 30-38 kD subunits. Mouse and human homologs of KLRG1 are expressed by subsets of NK cells and lymphokine-activated killer (LAK) cells but not mast cells. KLRG1 is also expressed on subsets of CD8+ and CD4+ cells, including CD4+ and CD8+ effector/memory cells, potent regulatory CD4+ T cells. KLRG1 may be involved in regulating NK cell homeostasis. KLRG1 was found to recognize cadherins and thus inhibit immune responses by regulating the effector function and the developmental processes of NK and T cells.
Product DetailsProduct Details
- Reactivity
- Mouse, Human
- Antibody Type
- Monoclonal
- Host Species
- Syrian Hamster
- Immunogen
- IL-2 activated NK cells from C57BL/6 mice
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
WB - Reported in the literature - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
For successful staining of human cells, brighter fluorophore-antibody conjugates, such as PE, BV421™, or APC, are recommended.
Additional reported applications (for relevant formats) include: Western Blotting1.
This product may be used for research purposes only. It is not licensed for resale and may only be used by the buyer. This product may not be used and is not licensed for clinical assays, where the results of such assays are provided as a diagnostic service. If a diagnostic or therapeutic use is anticipated, then a license must be requested from the University of California. The availability of such diagnostic and therapeutic use license(s) cannot be guaranteed from the University of California.
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Application References
(PubMed link indicates BioLegend citation) -
- Robbins SH, et al. 2002. J. Immunol. 168:2585. (WB)
- Robbins SH, et al. 2003. J. Immunol. 170:5876. (FC)
- McMahon CW, et al. 2002. J. Immunol. 169:1444. (FC)
- Product Citations
-
- RRID
-
AB_10578565 (BioLegend Cat. No. 138405)
AB_10575641 (BioLegend Cat. No. 138406)
Antigen Details
- Structure
- KLRG1 is an inhibitory lectin-like type II transmembrane receptor containing a cytoplasmic motif similar to ITIM, expressed mainly as a homodimeric molecule consisting of two N-glycosylated subunits of approximately 30-38 kD.
- Distribution
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Subsets of NK cells, lymphokine-activated killer (LAK) cells and subsets of CD8+ and CD4+ cells.
- Function
- Regulates NK cell proliferation, maturation, and homeostasis. Inhibits immune responses by regulating the effector function and the developmental processes of NK and T cells.
- Ligand Receptor
- E-cadherin
- Cell Type
- NK cells, T cells, Tregs
- Biology Area
- Immunology, Innate Immunity
- Antigen References
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1. Grundemann C, et al. 2006. J. Immunol. 176:1311.
2. Blaser C, et al. 1998. J. Immunol. 161:6451.
3. Huntington ND, et al. 2007. J. Immunol. 178:4764.
4. Voehringer D, et al. 2001. J. Immunol. 167:4834. - Gene ID
- 50928 View all products for this Gene ID
- UniProt
- View information about KLRG1 on UniProt.org
Related FAQs
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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