Biotin anti-mouse H-2Kk Antibody

Pricing & Availability
Clone
36-7-5 (See other available formats)
Other Names
MHC class I
Isotype
Mouse (A.TL) IgG2a, κ
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Product Citations
publications
36-7-5
C3H/He mouse splenocytes stained with 36-7-5 PE
  • 36-7-5
    C3H/He mouse splenocytes stained with 36-7-5 PE
  • 36-7-5 _b
    C3H/He mouse splenocytes stained with 36-7-5 PE
Cat # Size Price Quantity Avail. Save
114903 50 µg $70
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Description

The 36-7-5 antibody reacts with the H-2Kk MHC class I alloantigen expressed on nucleated cells from mice of the H-2Kk haplotype. H-2Kk is involved in antigen presentation to T cells expressing CD3/TCR and CD8 proteins. The 36-7-5 antibody does not react with alloantigens form other haplotypes (b, d, q), but weakly cross-reacts with splenocytes from SJL/Hsd mice.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse k haplotype, Weakly cross-reacts with SJL/Hsd mice splenocytes
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
A.AL mouse splenocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytotoxicity, and immunohistochemistry2,3 of acetone-fixed frozen sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 114914).

Does not react with alloantigens of other haplotypes (e.g., b, d, q).

Application References

(PubMed link indicates BioLegend citation)
  1. Goudeau B, et al. 2003. P. Natl. Acad. Sci. USA 100:15800.
  2. Johnson RM. 2004. Infect. Immun. 72:3951.
  3. Ardehali A, et al. 1995. Circulation 92:450.
Product Citations
  1. Ueno K, et al. 2016. Sci Rep. 6:28538. PubMed
Publication Library
RRID
AB_313610 (BioLegend Cat. No. 114903)

Antigen Details

Structure
MHC class I
Distribution
Nucleated cells of the H-2kk haplotype
Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD8
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.
3. Ian A, et al. 1996. Ann. Rev. Immunol. 14:369.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kk on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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