Biotin anti-mouse CCL28 (MEC) Antibody

Pricing & Availability
Clone
Poly5242 (See other available formats)
Other Names
Mucosa-associated epithelial chemokine (MEC), CCK1, SCYA28, small inducible cytokine A28
Isotype
Goat Polyclonal Ig
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Product Citations
publications
Poly5242_PURE_CCL28_Antibody_WB_050416
Western blot analysis of 50 ng of recombinant human CCL28 (lane 1) and 50 ng of recombinant mouse CCL28 (lane 2) using biotinylated anti-mouse CCL28 (clone Poly5242) antibody.
  • Poly5242_PURE_CCL28_Antibody_WB_050416
    Western blot analysis of 50 ng of recombinant human CCL28 (lane 1) and 50 ng of recombinant mouse CCL28 (lane 2) using biotinylated anti-mouse CCL28 (clone Poly5242) antibody.
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524203 50 µg $255
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Description

Mouse CCL28 was identified in an EST database using a human chemokine consensus sequence. CCL28 is constitutively expressed by mucosal epithelial cells such as exocrine glands, trachea, and colon. Acinar epithelial cells in human and mouse salivary glands are strongly positive for CCL28 by immunostaining, and human saliva and milk contain high concentrations of CCL28. Furthermore, it is known that human and mouse CCL28 have antimicrobial activity attributed to the C-terminus of CCL28 against Candida albicans, Gram-negative bacteria, and Gram-positive bacteria. In addition, using a CCR10 deficient mouse model, it was shown that CCR10 plays a critical role in localization and accumulation of IgA ASC in lactating mammary glands. Also, it has been described that CCL28 is upregulated in epithelial inflammation and this upregulation allows the recruitment of Tregs expressing CCR10. In fact, a subpopulation of CCR10-expressing CD25+ CD4+ Foxp3+ Tregs with potent anti-inflammatory properties was isolated from a chronically inflamed human liver. Information suggests that tumor hypoxia promotes the recruitment of Treg cells through induction of expression of CCL28, and this induction is mediated by the hypoxia inducible factor-1α (HIF-1α). Therefore, tumor hypoxia promotes tolerance and angiogenesis via CCL28 and Treg cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse CCL28
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.1-0.2 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of mouse CCL28 recombinant protein ranging from 1000 to 15.6 pg/mL are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5242 antibody is useful as the detection antibody in a sandwich ELISA assay, when used in conjunction with the purified L019H7 antibody (Cat. No. 525802) as the capture antibody and recombinant mouse CCL28 (Cat. No. 584709) as the standard.

RRID
AB_2563255 (BioLegend Cat. No. 524203)

Antigen Details

Structure
Chemokine
Distribution

Mucosal epithelial cells

Function
CCL28 chemoattracts resting CD4, CD8, and IgA antibody secreting cells. It plays a role in extravasation of IgA ASC in the small intestine, colon, lactating mammary glands, and has antimicrobial activity. CCL28 is upregulated by IL-1 or n-butirate in human colon epithelial cell lines, and IL-1 and n-butirate have a synergistic effect on CCL28 upregulation. Hypoxia induces CCL28 in tumors.
Interaction
CD4 and CD8 T cells, IgA antibody secreting cells, CCR10-expressing CD25+ CD4+ Foxp3+ Tregs
Ligand Receptor
CCR10, CCR3
Cell Type
Epithelial cells, Tregs
Biology Area
Angiogenesis, Cell Biology, Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Wang W, et al. 2000. J. Biol. Chem. 275:22313.
2. Hieshima K, et al. 2003. J. Immunol. 170:1452.
3. Hieshima K, et al. 2004. J. Immunol. 173:3668.
4. Ogawa H, et al. 2004. Am. J. Physiol. Gastrointest. Liver Physiol. 287:G1062.
5. Eksteen B, et al. 2006. J. Immunol. 177:593.
6. Morteau O, et al. 2008. J. Immunol. 181:6309
7. Liu B and Wilson E. 2010. Eur. J. Immunol. 40:186.
8. Facciabene A, et al. 2011. Nature 475:226.

Gene ID
56838 View all products for this Gene ID
UniProt
View information about CCL28 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 05/04/2016

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