Biotin anti-human/mouse IL-21 Antibody

Pricing & Availability
Clone
7H20-I19-M3 (See other available formats)
Other Names
Interleukin 21
Isotype
Mouse IgG1, κ
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Product Citations
publications
7H20-I19-M3_biotin_021711
Recombinant Human IL-21 (lane 1) and Mouse IL-21 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with biotin anti-HU/MU IL-21 antibody (clone 7H20-I19-M3). Proteins were visualized using a Streptavidin conjugated to HRP and chemiluminscence detection.
  • 7H20-I19-M3_biotin_021711
    Recombinant Human IL-21 (lane 1) and Mouse IL-21 (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with biotin anti-HU/MU IL-21 antibody (clone 7H20-I19-M3). Proteins were visualized using a Streptavidin conjugated to HRP and chemiluminscence detection.
  • 7H20-I19-M3_Biotin_IL21_Antibody_IF_100412
    Human PBMCs, stimulated with 1 µg/ml of LPS for 8 h and treated with Brefeldin A during the last 4 h, were prepared by cytospin, fixed and permeabilized on a slide and then treated with endogenous biotin blocking kit (Vector labs). Slides were stained with anti-human IL-21 biotin (clone 7H20-I19-M3) and DyLight™ 649 streptavidin (green) and counterstained with DAPI (blue). Images were acquired with an Olympus FV10i confocal microscope. Images courtesy of Teresa Rodriguez, Darius Schneider, and Matthias von Herrath, LaJolla Institute for Allergy and Immunology.
Cat # Size Price Quantity Avail. Save
514303 50 µg $90
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Description

Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset and has pleiotropic effects on both innate and adaptive immune responses.  These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells.  It can also be proapoptotic for B cells and NK cells.  Recent studies have shown that IL-21 is also an autocrine cytokine that potently induces T(H)17 differentiation and suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Cross-Reactivity: Mouse* - weak affinity
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant full length human IL-21
Formulation
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IF - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 5 µg antibody per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2125168 (BioLegend Cat. No. 514303)

Antigen Details

Distribution
Th17
Function
Immunomodulation
Cell Sources
NK-T cells and T cells
Cell Targets
cytotoxic T cells (CTL) and Natural Killer (NK) cells, that can destroy malignant or infected cells
Receptors
IL-21R
Cell Type
Th17
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Nurieva R,et al. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57.

Gene ID
59067 View all products for this Gene ID 60505 View all products for this Gene ID
UniProt
View information about IL-21 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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