Biotin anti-human IL-12/IL-23 p40 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
C8.6 (See other available formats)
Other Names
Interleukin-12 p40, Interleukin-23 p40, Cytotoxic lymphocyte maturation factor (CLMF), Natural killer cell stimulatory factor (NKSF), CTL maturation factor (TcMF), T-cell stimulating factor (TSF)
Isotype
Mouse IgG1, κ
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Product Citations
publications
Cat # Size Price Quantity Avail. Save
508801 50 µg $85
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508802 500 µg $230
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Description

The C8.6 antibody reacts with human IL-12 p40 subunit of the IL-12 p70 heterodimer and IL-23 p40 subunit of the IL-23 p19/p40, as well as p40 monomer and homodimer. The C8.6 antibody has been reported to strongly inhibit different biological activities of IL-12, (e.g., IFN-gamma induction, mitogenic effects on PHA blasts, and enhancement of NK cell-mediated cytotoxicity). The C8.6 antibody can neutralize the bioactivity of natural or recombinant IL-12.

Product Details
Technical data sheet

Product Details

Reactivity
Human, IL-12/IL-23 p40 subunit (monomer, homodimer and heterodimer IL-12 p35/p40 or IL-23 p19/p40)
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
COS-expressed p40 subunit of human IL-12
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

ELISA Detection, ELISPOT Detection

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay. For ELISA detection applications, a concentration range of 0.5-2.0 µg/ml is recommended.  To obtain a linear standard curve, serial dilutions of IL-12 p40 recombinant protein ranging from 4000 to 30 pg/ml are recommended for each ELISA plate.  To obtain a linear standard curve, serial dilutions of IL-12 p70 recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate.  For use as an ELISPOT detection antibody, a concentration range of 1-4 µg/ml is recommended.  It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA or ELISPOT Detection1,2: The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p40 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the C8.3 antibody (Cat. No. 501702/501704) as the capture antibody. The biotinylated C8.6 antibody is useful as a detection antibody for an IL-12 p70 specific sandwich ELISA or ELISPOT assay, when used in conjunction with the Purified 7B12 antibody (Cat. No. 511002/511004) as the capture antibody. Each lot of this antibody is quality control tested by ELISA assay.
Neutralization1,3,4: The LEAF™Purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for neutralization of human IL-12 bioactivity (Cat. No. 508804).

Application References

(PubMed link indicates BioLegend citation)
  1. D'Andrea A, et al. 1992. J. Exp. Med. 176:1387.
  2. D'Andrea A, et al. 1993. J. Exp. Med. 178:1041.
  3. Goodier M, et al. 2000. J. Immunol. 165:139.
  4. Aragane Y, et al. 1994. J. Immunol. 153:5366.
Product Citations
  1. Amici SA, et al. 2018. Front Immunol. 16:e2005090. PubMed
Publication Library
RRID
AB_315528 (BioLegend Cat. No. 508801)
AB_315529 (BioLegend Cat. No. 508802)

Antigen Details

Structure
Cytokine; monomer, heterodimer (p40:p35 or p40:p19) or homodimer (p40:p40)
Bioactivity
IL-12 p70 (p40:p35) induces IFN-γ, TNF-a production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. IL-23 (p40:p19) induces proliferation and production of IFN-γ by human me
Cell Sources
Dendritic cells, monocytes/macrophages, B cells, T cells
Cell Targets
T cells, NK cells, PBL
Receptors
IL-12Rβ1 binds p40; dimeric with IL-12Rβ2 binds p35
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
2. Quesniaux V. 1992. Research Immunol. 143:385.
3. Trinchieri G, et al. 1992 Prog. Growth Factor Res. 4:355.
4. Trinchieri G, et al. 1993 Immunol. Today. 14:335.
5. Oppmann B, et al. 2000 Immunity. 13:715.
6. Aggarwal S, et al. 2003 J. Biol. Chem. 278:1910.
7. Parham C, et al. 2002 J. Immunol. 168:5699.
8. Belladonna ML, et al. 2002 J. Immunol. 168:5448.
9. Lankford CS, et al. 2003 J. Leukoc. Biol. 73:49.

Regulation
Downregulated by IL-10; homodimeric p40 antagonistic to functional p70 heterodimer; p40 monomer has no function; p40 subunit in common with IL-23
Gene ID
3593 View all products for this Gene ID
UniProt
View information about IL-12 p40 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 2    Revision Date: 09/29/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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