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Biotin anti-human FcεRIα Antibody

Pricing & Availability
Clone
AER-37 (CRA-1) (See other available formats)
Regulatory Status
RUO
Other Names
FceRIa, FceRI-a, FceRI-alpha, FceRI alpha, high affinity IgE receptor
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
AER-37_Biotin_FceRIa_Antibody_1_032116
Human peripheral blood lymphocytes were stained with CD203c APC and biotinylated FcεRIα (clone AER-37) (top), or biotinylated mouse IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • AER-37_Biotin_FceRIa_Antibody_1_032116
    Human peripheral blood lymphocytes were stained with CD203c APC and biotinylated FcεRIα (clone AER-37) (top), or biotinylated mouse IgG2b, κ isotype control (bottom), followed by SAV-PE.
  • AER-37_Biotin_FceRIa_Antibody_2_032116
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334606 100 µg $253
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Description

High affinity IgE receptor (FcεRI) plays a key role in IgE-mediated allergic immune response. FcεRI is a tetrameric receptor complex, which is composed of one α-subunit (FcεRIα), one β-subunit, and two γ-subunits. FcεRIα directly binds IgE with high affinity, while the β- and γ-chains are responsible for mediating intracellular signals. FcεRIα is a 50 kD transmembrane protein with Ig superfamily structure. It is primarily found on mast cells and basophils. Further studies have indicated that FcεRIα is also expressed on many inflammatory cells including cutaneuos Langerhans cells, dendritic cells, monocytes of patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils produced in hypereosinophilic syndrome, and neutrophils from allergy-induced asthma patients.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone AER-37 (CRA-1) has been reported to bind the receptor even in the presence of IgE.4

Application References

(PubMed link indicates BioLegend citation)
  1. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
  2. Suzukawa M, et al. 2005. Int. Immunol. 17:1249.
  3. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  4. Yamaguchi M, et al. 1999. J. Immunol. 162:5455.
Product Citations
  1. Yang R, et al. 2020. Cell. 183(7):1826-1847.e31. PubMed
RRID
AB_2571885 (BioLegend Cat. No. 334606)

Antigen Details

Structure
Ig superfamily, 50 kD
Distribution

Mast cells, basophils, cutaneuos Langerhans cells, dendritic cells, and monocytes from the patients with allergic disorders, platelets, bronchial epithelial cells, eosinophils from hypereosinophilic syndrome, neutrophils from allergic asthmatic patients

Function
Bind IgE, trigger IgE-mediated allergic response
Ligand/Receptor
IgE
Cell Type
Basophils, Dendritic cells, Eosinophils, Langerhans cells, Mast cells, Monocytes, Neutrophils
Biology Area
Immunology
Molecular Family
Fc Receptors
Antigen References

1. Riske F, et al. 1991. J. Biol. Chem. 266:11245
2. Gounni AS, et al. 2001. FASEB J. 15:940.
3. Maurer D, et al. 1996. J. Immunol. 157:607
4. Maurer d, et al. 1994. J. Exp. Med. 179:745
5. Campbell AM, et al. 1998. Am. J. Respir. Cell Mol. Biol. 19:92.

Gene ID
2205 View all products for this Gene ID
UniProt
View information about FcepsilonRIalpha on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 03/24/2016

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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