Biotin anti-human CD286 (TLR6) Antibody

Pricing & Availability
Clone
TLR6.127 (See other available formats)
Other Names
TLR6, CD286
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
TLR6dot127_Biotin_082808.jpg
Human peripheral blood monocytes stained with biotinylated TLR6.127, followed Sav-PE
  • TLR6dot127_Biotin_082808.jpg
    Human peripheral blood monocytes stained with biotinylated TLR6.127, followed Sav-PE
Cat # Size Price Quantity Avail. Save
334705 25 µg $105
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Description

Toll-like receptors (TLRs) are type I transmembrane signaling receptors characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain. TLRs are responsible for recognizing pathogen-associated molecule patterns (PAMPs). They are primary sensors of both innate and adaptive immune systems. To date a total of 12 TLRs have been identified. Toll-like receptor 6 (TLR6), known as CD286, is one of the important receptors. TLR6 is colocalized with TLR2 on the cell surface of monocytes/macrophages, dendritic cells, granulocytes. The TLR2/TLR6 complex plays a role in the innate immune response to diacylated lipoprotein and peptidoglycan antigens.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human TLR6 transfected Ba/F3 cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: blocks cytokine production induced by MALP-2 and peptidoglycan(1)

Application References

(PubMed link indicates BioLegend citation)
  1. Nakao Y, et al. 2005. J. Immunol. 174:1566
RRID
AB_1186049 (BioLegend Cat. No. 334705)

Antigen Details

Structure
Type I transmembrane protein characterized by containing leucine-rich repeats (LRR) extracellular domain and IL-1 receptor like intracellular domain, colocalized with TLR2 at the cell surface
Distribution
Monocytes/macrophages, dendritic cells, granulocytes
Function
Activates innate host defense against diverse set of pathogens
Ligand/Receptor
Diacylated lipoprotein, peptidoglycan
Cell Type
Monocytes, Macrophages, Dendritic cells, Granulocytes
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Toll Like Receptors
Antigen References

1. Takeuchi O, et al. 2001. Int. Immunol. 13:933
2. Shimizu T, et al. 2005. J. Immunol. 175:4641
3. Into T, et al. 2004. Cell Microbiol. 6:187

Gene ID
10333 View all products for this Gene ID
UniProt
View information about CD286 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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