Biotin anti-human CD202b (Tie2/Tek) Antibody

Pricing & Availability
Clone
33.1 (Ab33) (See other available formats)
Other Names
Tie2, TEK
Isotype
Mouse IgG1 κ
Ave. Rating
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Product Citations
publications
Ab33_Biotin_082808.jpg
Human endothelial cell line, HUVEC, stained with biotinylated 33.1, followed by Sav-PE
  • Ab33_Biotin_082808.jpg
    Human endothelial cell line, HUVEC, stained with biotinylated 33.1, followed by Sav-PE
Cat # Size Price Quantity Avail. Save
334204 100 µg $180
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Description

CD202b is a 145 kD type I transmembrane protein, also known as Tie2 or TEK. It is a member of the receptor tyrosine kinase (RTK) family of proteins and is expressed by endothelial cells and their progenitors, quiescent hematopoietic stem cells (HSCs), and a subpopulation of monocytes. Angiopoietin-1 (Ang-1) is an activator of CD202b to promote, maintain, and stabilize mature vessels and to maintain HSCs in quiescent state. Ang-2 is another ligand of CD202b, which is involved in postnatal angiogenesis and in antagonizing the effects of Ang-1. Tie2 binds to Ang-4 as well.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant extracellular domain of human Tie2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications include: immunoprecipitation, Wstern blot, immunohistochemical staining of frozen tissue sections and ELISA

Application References

(PubMed link indicates BioLegend citation)
  1. Peters KG, et al. 1998. Br. J. Cancer. 77:51.
  2. Wong AL, et al. 1997. Circ. Res. 81:567.
  3. Lin P, et al. 1998. P. Natl. Acad. Sci. USA 95:8829.
RRID
AB_1186046 (BioLegend Cat. No. 334204)

Antigen Details

Structure
145 kD type I transmembrane protein, receptor tyrosine kinase (RTK) family
Distribution
Endothelial cells, subsets of hematopoietic stem cells and and a subset of monocytes
Function
Angiogenesis, hemotopoiesis, monocytes chemotaxis
Ligand Receptor
Angiopoietin-1 (Ang-1), Ang-2, and Ang-4
Cell Type
Endothelial cells, Hematopoietic stem and progenitors, Monocytes
Biology Area
Angiogenesis, Cell Biology, Immunology
Molecular Family
CD Molecules
Antigen References

1.De Palma M, et al. 2005. Cancer Cell. 8:211
2.Shaw JP, et al. 2004. Blood Cells Mol. Dis. 32:168
3. Hsu HC, et al. 2000. Blood. 96:3757
4. Arai F, et al. 2004. Cell. 118:149

Gene ID
7010 View all products for this Gene ID
UniProt
View information about CD202b on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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