Biotin anti-Connexin 43, 360-382 Antibody

Pricing & Availability
Clone
P2C4 (See other available formats)
Other Names
GJA1, Cx43, Gap Junction Protein Alpha 1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
P2C4_Biotin_Connexin_Antibody_1_090418
Western blot of Biotin anti-Connexin 43, 360-382 antibody (clone P2C4). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 5 μg of mouse brain lysate; Lane 4: 5 μg of rat brain lysate. The blot was incubated with 0.1 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • P2C4_Biotin_Connexin_Antibody_1_090418
    Western blot of Biotin anti-Connexin 43, 360-382 antibody (clone P2C4). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 5 μg of mouse brain lysate; Lane 4: 5 μg of rat brain lysate. The blot was incubated with 0.1 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP Streptavidin (Cat. No. 405210). Enhanced chemiluminescence was used as the detection system.
  • P2C4_Biotin_Connexin_Antibody_2_090418
    IHC staining of Biotin anti-Connexin 43, 360-382 antibody (clone P2C4) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • P2C4_Biotin_Connexin_Antibody_3_090418
    IHC staining of Biotin anti-Connexin 43, 360-382 antibody (clone P2C4) on formalin-fixed paraffin-embedded mouse heart tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • P2C4_Biotin_Connexin_Antibody_4_090418
    IHC staining of Biotin anti-Connexin 43, 360-382 antibody (clone P2C4) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928602), the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. For detection, the HRP labeling reagent and DAB from BioLegend Ultra Streptavidin (USA) HRP Detection Kit was used (Multi-Species, component #5, DAB; Cat. No. 929901). Slides were counterstained with hematoxylin, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
Cat # Size Price Quantity Avail. Save
849605 25 µg $120
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849606 100 µg $270
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Description

Connexins are a family of transmembrane proteins that assemble to form vertebrate gap junctions, thus also called Gap Junction Proteins. So far, 21 Connexins have been discovered in humans. The gene GJA1 encodes Connexin 43 (Cx43), which is the most expressed Connexin, and can be found in many human tissues including skin, bone, and brain. Cx43 has been reported to be involved in synchronized contraction of the heart as well as embryonic development. Besides its functions as a channel protein, Cx43 also performs channel-independent functions. During brain and heart development, cytoplasmic Cx43 regulates the microtubule network and thus affects cell migration and polarity. In addition, mitochondrial GJA1 has also been reported to promote cell survival by down-regulating the intrinsic apoptotic pathway during conditions such as oxidative stress.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH-conjugated peptide corresponding to amino acids 360-382 of Connexin 43.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
IHC-P - Validated

Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 0.1 µg per ml. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This antibody binds to the C-terminus of Connexin 43 and shows preference for non-phosphorylated Connexin 43 by western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Chu G, et al. 2002. Cardiovasc. Res. 54(1):105. (WB)
  2. Wei CJ, et al. 2005. J. Biol. Chem. 280(20):19925. (WB)
RRID
AB_2750331 (BioLegend Cat. No. 849605)
AB_2750332 (BioLegend Cat. No. 849606)

Antigen Details

Structure
Connexin 43 (GJA1) is a 382 amino acid protein with a molecular mass of 43 kD.
Distribution

Tissue distribution: Expressed in most tissues, enriched in brain, endocrine, muscle, bone marrow, immune System, ovaries, testes, and Skin.

Cellular distribution: Plasma membrane, cytosol, mitochondria, golgi apparatus, cytoskeleton and endoplasmic reticulum membrane.

Function
Connexin 43 plays a role in cell-to-cell communication by forming channels, or gap junctions between cells. It allows for the transport of nutrients, charged particles (ions), and other small molecules that carry necessary communication signals between cells.
Molecular Family
Gap Junction Proteins
Antigen References

1. Grek CL, et al. 2016. Cancer Lett. 374(1):117.
2. Lilly E, et al. 2016. Semin. Cell Dev. Biol. Feb; 50:4
3. Michela P, et al. 2015. Eur. J. Pharmacol. 768:71-6.

Gene ID
2697 View all products for this Gene ID
UniProt
View information about Connexin 43 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 1    Revision Date: 09/04/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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