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Biotin anti-c-Myc Antibody (Previously Covance catalog# BIOT-150L)

Pricing & Availability
Clone
9E10 (See other available formats)
Regulatory Status
RUO
Other Names
myc tag, c-Myc tag, cMyc tag, Myc epitope tag
Previously
Covance Catalog# BIOT-150L
Isotype
Mouse IgG1, κ
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Product Citations
publications
9E10_c-myc_epitope_tag_1_011918
Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respectively) of Posi-tag (Cat. No. 931301) using Biotin anti C-Myc Mouse primary antibody (Clone 9E10, 1:2500 dilution, 0.4 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 9E10_c-myc_epitope_tag_1_011918
    Western blot analysis of 1, 0.5, 0.1 µl (lanes 1, 2, 3 respectively) of Posi-tag (Cat. No. 931301) using Biotin anti C-Myc Mouse primary antibody (Clone 9E10, 1:2500 dilution, 0.4 µg/ml) and HRP Avidin secondary antibody (Cat. No. 405103, 1:1000 dilution).
  • 9E10_c-myc_epitope_tag_2_011918
    Immunofluorescence of Myc-tagged plasmid-transfected A549 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) Biotin-Myc Mouse primary antibody (Clone 9E10). Alexa Fluor® 594 (Red) Streptavidin (Cat. No. 405240) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/30, (B, D) is 1/600 and (C) is 1/900. Concentrations for (A-C) is 1 µg/ml, (D) is 0.5 µg/ml.
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908805 100 µL $419
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Description

The 9E10 monoclonal antibody recognizes the amino acid sequence EQKLISEEDL, which is a specific portion of the human c-myc gene product. This antibody is highly specific and is unlikely to alter the activity of the cloned sequence.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Epitope tag
Reported Reactivity
Species independent
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Amino acids 408-439, C-terminal region of human c-myc
Formulation
Phosphate-buffered solution + 0.03% Thimerosal + 50% Glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application

WB - Quality tested
ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.4 µg per ml (1:2500 dilution). For immunocytochemistry, a concentration range of 0.5 - 1.0 μg/ml (1:1000-1:2000) is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

*Our Posi-Tag Control Protein (931301) can be used as a helpful positive control for this antibody.

Application References

(PubMed link indicates BioLegend citation)
  1. Rao H, et al. 2001. Nature. 410:955.
  2. Peters CS, et al. 2001. J Biol Chem. 276:13718.
  3. Munro S, Pelham HR. 1987. Cell. 48:899.
  4. Evan GI, et al. 1985. Mol Cell Biol. 5:3610.
Product Citations
  1. Rad S M AH, et al. 2020. PLoS One. 15:e0232915. PubMed
  2. Marshall RS, et al. 2020. J Cell Sci. 133:jcs249862. PubMed
  3. Parodi E, et al. 2015. PLoS One. 10: 0143571. PubMed
  4. Marshall RS, et al. 2020. J Cell Sci. :133. PubMed
RRID
AB_2565090 (BioLegend Cat. No. 908805)

Antigen Details

Biology Area
Cell Biology, Protein Purification, Protein Synthesis
Antigen References
  1. Adams JM, et al. 1983. Proc. Natl. Acad. Sci. USA 80:1982.
  2. Atchley WR, et al. 1995. Proc. Natl. Acad. Sci. USA 92:10217.
  3. Battey J, et al. 1983. Cell 34:779.
  4. Beimling P, et al. 1985. Biochemistry 24:6349.
Gene ID
4609 View all products for this Gene ID
UniProt
View information about c-Myc on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 10    Revision Date: 10/03/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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