Biotin anti-β-Amyloid, 1-38 Antibody (Previously Covance catalog# MRB-5215)

Pricing & Availability
Clone
7-14-4 (See other available formats)
Other Names
AAA, ABETA, ABPP, AD1, APPI, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
Previously
Covance Catalog# MRB-5215
Isotype
Rabbit IgG
Ave. Rating
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Product Citations
publications
7-14-4_Biotin_Beta_Amyloid_Antibody_ELISA_081115
Direct ELISA showing specificity of Beta Amyloid 1-38 (7-14-4), Clone 7-14-4), on 1-38 (+ ctrl) and 1-40 (- ctrl) peptide coated plates.
  • 7-14-4_Biotin_Beta_Amyloid_Antibody_ELISA_081115
    Direct ELISA showing specificity of Beta Amyloid 1-38 (7-14-4), Clone 7-14-4), on 1-38 (+ ctrl) and 1-40 (- ctrl) peptide coated plates.
Cat # Size Price Quantity Avail. Save
808603 50 µl $405
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Description

Soluble Aβ (Amyloid-beta, beta-amyloid, or Abeta) peptides, 36 to 43 amino acids in length, are natural proteolytic cleavage products of amyloid precursor protein (APP). However, soluble abnormally aggregated Aβ species are associated with Alzheimer's disease (AD), can be toxic to neurons, and insoluble Aβ aggregates are the main component of cerebral amyloid plaques, which are a pathological hallmark of AD.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rabbit
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
Application

ELISA, WB, IHC
IF - Reported in the literature

Recommended Usage

Each lot of this antibody is quality control tested by ELISA assay.

The optimal working dilution should be determined for each specific assay condition.
WB: 1:1,000 - 1:2,000*
IHC: 1:50 - 1:100
Tissue: Formalin-fixed Paraffin-embedded Tissues
Pre-treatment: 70% Formic Acid for 20 minutes at room temperature
Primary incubation: 24 hours at 2-4°C

ELISA: Can be used as capture antibody in a sandwich ELISA with 4G8 detection (Ref Cat# 800707 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody, HRP Labeled, or Cat No. 800701 Beta-Amyloid, 17-24 (4G8) Monoclonal Antibody).
For capture antibody, recommended usage is between 1.0 and 10.0 µg/ml.
Recommended blocking buffer is 0.5-1.0% BSA in PBS.
If using Cat No. 800707 for detection; recommended usage is between 0.25-1.0 µg/ml.

Application Notes

This antibody is effective in immunoblotting (WB), immunohistochemistry (IHC), and ELISA.

*Expected MW: 4 kD

WB Positive Control: Alzheimer's Brain Lysate

IHC Positive Control: Alzheimer's Brain Tissue

This antibody is reactive to the C-terminus of beta amyloid and is specific for the isoform that ends at the 38th amino acid.

Application References

(PubMed link indicates BioLegend citation)
  1. Watts JC, et al. 2014. Proc. Natl. Acad. Sci. USA 28:10323. ((F)
  2. Giaccone G, et al. 2012. Acta. Neuropathol. 124:809. (IHC, IF, WB)
RRID
AB_2564746 (BioLegend Cat. No. 808603)

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid, 1-38 on UniProt.org

Related FAQs

How many biotin molecules are per antibody structure?
We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
Go To Top Version: 4    Revision Date: 05/31/2016

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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