- 4B12/Synuclein (See other available formats)
- Other Names
- NACP, PARK1, PARK4, PD1, Synuclein alpha-140, non-A4 component of amyloid, alpha-synuclein, isoform NACP140, non-A beta component of AD amyloid Parkinson disease
- Mouse IgG1, κ
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- Product Citations
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α-Synuclein is expressed principally in the nervous system, but it is also produced in other tissues, including the skin. In the brain, the protein is primarily neuronal, but it is also present in glia. Neuronal α-synuclein is concentrated in presynaptic nerve terminals, interacts with plasma membrane phospholipids, and is also present in nuclei and mitochondria. At least three isoforms of synuclein are produced through alternative splicing. The most common isoform is a 140 amino acid-long transcript. Other isoforms are a-synuclein-126, lacking residues 41-54; and α-synuclein-112, which lacks residues 103-130. α-Synuclein’s physiological role is poorly understood, but the protein has been implicated in regulating dopamine release and transport, synaptic vesicle clustering, and functioning as a SNARE-complex chaperone. α-Synuclein fibrils are major component of the intracellular Lewy bodies that are associated with Parkinson's disease, Lewy body dementia, and multiple system atrophy.Product Details
- Antibody Type
- Host Species
- This antibody was developed using a purified, E. coli produced human α-synuclein.
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- The antibody was purified by affinity chromatography and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
IHC-P - Quality tested
WB, ELISA Detection - Validated
- Recommended Usage
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For ELISA Detection applications, a concentration range of 0.5 -2.0 µg/mL is recommended. To obtain a linear standard curve, serial dilutions of α-synuclein recombinant protein ranging from 6 to 1500 pg/mL is recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
This antibody reacts with human α-synuclein. It does not react with mouse or rat α-synuclein. The epitope lies within amino acids 103-108 of α-synuclein.
Additional reported applications (for the relevant formats) include: ELISA2, Western blotting1,2,3,4,6,8, and immunoprecipitation4.
(PubMed link indicates BioLegend citation)
- Lahut S, et al. 2017. Dis Model Mech. (WB) PubMed
- Landeck N, et al. 2016. Mol Neurodegener. 11(1): 61. (ELISA, WB) PubMed
- Moussaud S, et al. 2015. Expert Opin Ther Targets. 19(5): 589. (WB) PubMed
- Helferich AM., et al. 2015. Mol Neurodegener. 10(1): 66. (IHC, IP, WB) PubMed
- Bodis‐Wollner I, et al. 2014. Ann Neurol. 75(6): 964. (IHC)
- Shen W, et al. 2014. J Neurochem. 129(5): 884. (WB)
- Fagerqvist T, et al. 2013. J Neurochem. 126(1): 131. (IHC)
- Ebrahimi-Fakhari D, et al. 2011. J Neurosci. 31(41): 14508. (WB) PubMed
- Welander H, et al. 2011. Biochem Biophys Res Commun. 412(1): 32. (IHC)
AB_2721318 (BioLegend Cat. No. 807807)
AB_2721319 (BioLegend Cat. No. 807808)
- α-Synuclein’s canonical isoform is a 140 amino acid protein with an apparent molecular mass of 14 kD.
Tissue distribution: Primarily nervous system, but lower expression in other tissues.
Cellular distribution: Cytoskeleton, cytosol, lysosome, mitochondria, nucleus, plasma membrane, and extracellular.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- Antigen References
- Lee JM, et al. 2017. J Neuropathol Exp Neurol. 76(1):2.
- Kang SS, et al. 2017. Proc Natl Acad Sci. 114(5): 1183.
- Valdes P, et al. 2016. Front Neuroanat. 10:123.
- Emamzadeh FN. 2016. J Res Med Sci. 9(21):29.
- Gene ID
- 6622 View all products for this Gene ID
- View information about α-Synuclein, 103-108 on UniProt.org
- How many biotin molecules are per antibody structure?
- We don't routinely measure the number of biotins with our antibody products but the number of biotin molecules range from 3-6 molecules per antibody.
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