APP/Aβ Antibody Sampler Kit

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APPslashAB_Antibody_Sampler_Kit_1_120718
IHC staining of purified anti-β-Amyloid, 1-16 antibody (clone 6E10) on the formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • APPslashAB_Antibody_Sampler_Kit_1_120718
    IHC staining of purified anti-β-Amyloid, 1-16 antibody (clone 6E10) on the formalin-fixed paraffin-embedded normal human (left panel) and Alzheimer’s disease (right panel) brain tissues. Following antigen retrieval using 70% formic acid for 20 minutes, the tissues were incubated with 1 µg/mL of the primary antibody for 1 hour at room temperature. BioLegend’s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • APPslashAB_Antibody_Sampler_Kit_2_120718
    Western blot of purified anti-mouse/rat β-Amyloid, 1-16 antibody (clone M3.2). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain total lysate; Lane 3: 20 µg of mouse brain total lysate; Lane 4: 20 µg of rat brain total lysate. The blot was incubated with 0.025 µg/mL of the primary antibodies overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405301). Enhanced chemiluminescence was used as the detection system.
  • APPslashAB_Antibody_Sampler_Kit_3_120718
    IHC staining of purified anti-β-Amyloid, 1-40 antibody (clone 11A50-B10) on formalin-fixed paraffin-embedded Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • APPslashAB_Antibody_Sampler_Kit_4_120718
    IHC staining of the purified anti-β-Amyloid, 1-42 antibody (12F4) on the formalin-fixed paraffin-embedded brain tissues from normal human (left panel) and patients with Alzheimer’s disease (left panel) and normal human brain tissue (right panel). Following antigen retrieval using 70% formic acid for 20 min, the tissue was incubated overnight with 1µg/mL of the primary antibody at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • APPslashAB_Antibody_Sampler_Kit_5_120718
    Western blot of the purified anti-β-amyloid, 1-42 antibody (clone 12F4). Lane 1: Molecular weight marker; Lane 2: 50 ng of the human Aβ1-40 peptide; Lane 3: 50 ng of the Aβ1-42 peptide. The blot was incubated with 1 µg/ml of the primary antibody overnight at 4°C, followed by incubation with the HRP-labeled goat anti-mouse IgG (cat: 405306). Enhanced chemiluminescence was used as the detection system.
  • APPslashAB_Antibody_Sampler_Kit_6_120718
    IHC staining of purified anti-β-Amyloid, 1-15 antibody (clone 3A1) on frozen normal (left panel) and Alzheimer's disease (right panel) human brain tissues. The tissues were incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Detection Kit (Cat. No. 929901) was used for detection followed by hematoxylin and bluing solution counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm.
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899910 1 kit $415
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Description

The Aβ Antibody Sampler Kit offers flexibility for sampling and detection of full-length amyloid precursor protein (APP) and various APP cleavage fragments including Aβ 1-40 and Aβ 1-42 peptides. The kit also includes clone 3A1, which offers unique advantages over gold standard antibodies for Aβ detection such as clones 6E10 and 4G8. In addition to detecting C-terminally processed fragments of APP, clone 3A1 shows preference for aggregated Aβ and demonstrates no cross-reactivity with APP. Clone M3.2 recognizes rodent Aβ, β-CTF, sAPP-α, and full-length APP. Clone M3.2 has negligible cross-reactivity with human isoforms of APP and Aβ. The antibodies provided in this kit are highly suitable for WB, IHC, and ELISA applications.

Product Details
Technical data sheet

Kit Contents

Kit Contents
Specificity Clone Size Reactivity Isotype
Anti-β-Amyloid, 1-16 6E10 25 μl Human Mouse IgG1
Anti-mouse/rat β-Amyloid, 1-16 M3.2 25 μl Mouse, Rat Mouse IgG1, κ
Anti-β-Amyloid, 1-40 11A50-B10 50 μl Human Mouse IgG1
Anti-β-Amyloid, 1-42 12F4 50 μl Human, Mouse, Rat Mouse IgG1
Anti-β-Amyloid, 1-15 3A1 25 μg Human Mouse IgG1, κ

* For detailed information about each specificity, please refer to the datasheets of the individual products. 

Product Details

Antibody Type
Monoclonal
Formulation
Please refer to individual product datasheets of the purified formats for details.
Preparation
All antibodies in this kit were purified by affinity chromatography.
Storage & Handling
Upon receipt, store undiluted at 2-8°C.
Application

WB, IHC-P, IHC-F - Quality tested

Recommended Usage

Each lot of antibodies in this kit is quality control tested by Western blotting. For Western blotting, the suggested uses of these reagents are as follows:

Anti-β-Amyloid, 1-16: 0.2 - 1.0 µg/ml
Anti-mouse/rat β-Amyloid, 1-16: 0.1 - 5.0 µg/ml

For immunohistochemistry on formalin-fixed paraffin-embedded tissue, the suggested use of this reagent is as follows:

Anti-β-Amyloid, 1-40: 5.0 - 10 µg/ml
Anti-β-Amyloid, 1-42: 0.2 - 5.0 µg/ml

For immunohistochemistry on frozen tissue, the suggested use of this reagent is as follows:

Anti-β-Amyloid, 1-15: 1.0 - 5.0 µg/ml

It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For validated or reported applications for these antibodies, please see individual product datasheets.

Antigen Details

Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Gene ID
351 View all products for this Gene ID

Related FAQs

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Go To Top Version: 1    Revision Date: 12/07/2018

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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