Apotracker™ Green

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Apotracker_Green_1_101819
One day old splenocytes were stained with 400nM Apotracker™ Green and Annexin V Brilliant Violet 421™ in Annexin V staining buffer.
  • Apotracker_Green_1_101819
    One day old splenocytes were stained with 400nM Apotracker™ Green and Annexin V Brilliant Violet 421™ in Annexin V staining buffer.
  • Apotracker_Green_2_101819
    One day old splenocytes were stained with 400nM Apotracker™ Green and Helix NP™ NIR (Cat. No. 425301) in Cell Staining Buffer.
  • Apotracker_Green_3_100919
    HeLa cells were cultured for 3 days on a chamber slide coated with poly-d-lysine and then stained with Apotracker™ Green 800nM, Calcein Red-AM 0.5µM, and Helix NP™ Blue 5nM (Cat. No. 425305). Apoptotic cells (green) are Apotracker™ Green positive, live healthy cells (red) are Calcein Red-AM positive, and dead cells (blue) are Helix NP™ Blue positive.
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Cat # Size Price Quantity Avail. Save
427401 20 tests $45
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427402 100 tests $210
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427403 200 tests $400
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Description

Apotracker Green is a calcium-independent probe for detecting apoptotic cells. Similar to Annexin V, it detects the translocation of phosphatidylserine residues to the cell surface in a cell undergoing apoptosis. This probe can be used in conjunction with a dead cell indicator like the Helix impermeant nucleic acid stains or Zombie live/dead probes in regular cell staining buffer or PBS.

Product Details
Technical data sheet

Product Details

Formulation
This product consist of dry-down Apotracker™ Green and DMSO reconstitution solution.
Preparation
Apotracker™ Green is prepared by dissolving the probe in methanol and drying it down.
Storage & Handling
Store Apotracker™ Green at -20°C
Application

FC - Quality tested
Live cell imaging - Validated

Recommended Usage

Each lot of this product is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 200-800 nM per million cells in 100 µl volume. For live cell imaging, a concentration range of 600 nM-1.0 μM is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Apotracker™ Green excites at 500 nm and emits at 520 nm. The bandpass filter 530/30 is recommended for detection, although filter optimization may be required depending on other fluorophores used. Be sure to verify that your cytometer configuration and software setup are appropriate for detecting this channel. Refer to your instrument manual or manufacturer for support. The emission spectra of Apotracker™ Green is nearly identical to FITC and Alexa Fluor® 488. Therefore, we do not recommend to use them in the same panel in conventional and spectral flow cytometry.

Application Notes

To reconstitute the reagent to an 80 µM stock concentration, add the following volumes:

  • Add 200 µL DMSO to the 200 test size
  • Add 100 µL DMSO to the 100 test size
  • Add 20 µL DMSO to the 20 test size

 

  1. Take the appropriate volume of reconstitute Apotracker solution to perform a 1:10 dilution with cell staining buffer.
  2. Add 5 µL of the diluted reagent to 1x106 cells in 100 µL of an appropriate buffer, like FACS staining buffer, to achieve a 400nM staining solution. However, different cell types might require optimization of the staining solution concentration. We recommend a 200-800nM range for cells in suspension.
  3. Incubate at room temperature for 10-20 minutes.
  4. Co-stain with an appropriate dead cell stain as needed.
  5. Wash the cells at least 2 times prior to running on the cytometer. This reagent will be registered in the FITC channel of the flow cytometer.

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Proliferation and Viability
Gene ID
NA

Related FAQs

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Go To Top Version: 1    Revision Date: 10/09/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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