APC/Cyanine7 anti-mouse I-A/I-E Antibody

Pricing & Availability
Clone
M5/114.15.2 (See other available formats)
Other Names
MHC class II
Isotype
Rat IgG2b, κ
Ave. Rating
3 reviews
Product Citations
publications
M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
  • M5-114-15-2_APCCyanine7_IA-IE_Antibody_020119
    C57BL/6 mouse splenocytes were stained with anti-mouse CD11b FITC and I-A/I-E (clone M5/114.15.2) APC/Cyanine7 (left) or Rat IgG2b, κ APC/Cyanine7 isotype control (right).
Cat # Size Price Quantity Avail. Save
107627 25 µg $85
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107628 100 µg $265
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Description

These class II molecules are expressed on antigen presenting cells (including B cells) and a subset of T cells from H-2b,d,q,r bearing mice and are involved in antigen presentation to T cells expressing CD3/TCR and CD4 proteins.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Activated C57BL/6 mouse spleen cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions. The solution is free of unconjugated APC/Cyanine7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The M5/114.15.2 antibody reacts with a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek MHC class II alloantigens from mice carrying H-2p,r,q,b,d,u haplotypes. Clone M5/114.15.2 however does not react wtih I-Af, I-Ak, or I-As MHC class II alloantigens.1

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of frozen sections2,3,6, and in vitro and in vivo blocking of antigen presentation or ligand binding4-7. The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. Nos. 107655 & 107656).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Bhattacharya A, et al. 1981. J. Immunol. 127:2488. (IP)
  2. Viville S, et al. 1993. Cell 72:635. (IHC)
  3. Nelson AJ, et al. 1993. J. Immunol. 151:2453. (IHC)
  4. Shi Y, et al. 1998. J. Exp. Med. 187:367. (Block)
  5. Yamashita I, et al. 1993. Int. Immunol. 5:1139.
  6. Guo M, et al. 1995. Zygote 3:65. (IHC)
  7. Kim A, et al. 2004. Exp. Mol. Med. 36:428. (Block)
  8. Luckashenak NA, et al. 2006. J. Immunol. 177:5177.
  9. Venanzi ES, et al. 2007. J. Immunol. 179:5693.
  10. Christensen SR, et al. 2006. Immunity 25:417. PubMed
  11. Matte-Martone C, et al. 2008. Blood 111:3884. PubMed
  12. De Pascalis R, et al. 2008. Infect. Immun. 76:4311. PubMed
  13. Kuns RD, et al. 2009. Blood 113:5999. PubMed
  14. Sabatino JJ, et al. 2011. J. Exp. Med. 208:81. PubMed
  15. Draber P, et al. 2011. Mol Cell Biol. 22:4550. PubMed
  16. Fu H, et al. 2014. Nat Commun. 5:3436. PubMed
Product Citations
  1. Henningsson L, et al. 2010. Infect Immun. 78:3785. PubMed
  2. Céspedes P, et al. 2014. Proc Natl Acad Sci U S A. 111:3214. PubMed
  3. Schwartz C, et al. 2014. Proc Natl Acad Sci U S A. 111:5169. PubMed
  4. Terrazas M 2014. Int J Parasit. 44 613 . PubMed
  5. Castillo V, et al. 2015. PLoS One. 10: 0136620. PubMed
  6. Engel O, et al. 2015. Stroke. 46: 3232 - 3240. PubMed
  7. Lim S, et al. 2016. PLoS One. 11: 0155689. PubMed
  8. Ghazal R, et al. 2016. Neoplasia. 18: 294-306. PubMed
  9. Hilpert C, et al. 2016. J Immunol. 197: 2780 - 2786. PubMed
  10. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  11. Chandra J, et al. 2016. Immunol Cell Biol. 10.1038/icb.2016.83. PubMed
  12. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  13. Bellelli R 2018. Molecular cell. 70:707. PubMed
  14. Ding L, et al. 2018. Cell Rep. 25:2972. PubMed
  15. Lancaster JN, et al. 2019. Nat Commun. 10:2220. PubMed
  16. Li J, et al. 2019. iScience. 16:468. PubMed
  17. Kitaura F, et al. 2019. PLoS One. 14:e0220199. PubMed
Publication Library
RRID
AB_1659252 (BioLegend Cat. No. 107627)
AB_2069377 (BioLegend Cat. No. 107628)

Antigen Details

Structure
MHC class II
Distribution

B cell and activated T cells, APCs of the H-2b,d,q,r bearing mice

Function
Antigen presentation
Ligand/Receptor
CD3/TCR, CD4
Cell Type
Antigen-presenting cells, B cells, Dendritic cells, T cells, Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Watts C. 1997. Ann. Rev. Immunol. 15:821.
2. Pamer E, et al. 1998. Ann. Rev. Immunol. 16:323.

Gene ID
14961 View all products for this Gene ID 14969 View all products for this Gene ID
UniProt
View information about I-A/I-E on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 5    Revision Date: 02/01/2019

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