APC Streptavidin

Pricing & Availability
Regulatory Status
RUO
Other Names
Streptavidin-Allophycocyanin, SAv-APC
Ave. Rating
Submit a Review
Product Citations
publications
Cat # Size Price Quantity Avail. Save
405207 100 µg $100
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Streptavidin binds to biotin with high affinity. Streptavidin-APC is useful for detecting biotinylated antibodies. The excitation of APC by 600-635 nm laser light induces a fluorescence emission maximum of 660 nm.

Product Details
Technical data sheet

Product Details

Reactivity
Human, Mouse, Rat, All Species
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
Streptavidin is conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml (concentration relates to the Streptavidin only component of the conjugate)
Storage & Handling
The Streptavidin-APC solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC, ICFC - Quality tested

Recommended Usage

Each lot of this Streptavidin-APC is quality control tested by immunofluorescent staining with flow cytometric analysis. The concentration provided is based upon molecular mass of streptavidin independent of any additional molecular mass that might be added by the APC conjugation. For immunofluorescent staining, the recommended use of this reagent is ≤ 0.125 µg per 106 cells in 100 µl staining volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Streptavidin-APC is useful as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. The average molecular weight of Streptavidin-APC is 320 kD and Streptavidin alone is 52 kD.

Application References

(PubMed link indicates BioLegend citation)
  1. Kamimura D, et al. 2006. J. Immunol. 177:306.
  2. Andoniou CE, et al. 2005. Nature Immunology 6:1011.
  3. Chou WC,et al.2006.Blood108:3005. PubMed
  4. Malagolini N, et al. 2009. Glycobiology. 19:172. PubMed
  5. Kroeger KM, et al. 2009. J. Leukocyte Biol. PubMed
  6. Imada M, et al. 2009. Int. Immunol. 21:1151. PubMed
  7. Yi Y, et al. 2010 J. Biol Chem. 285:21233. PubMed
  8. King IL, et al. 2010. J. Immunol. 185:6138. PubMed
  9. Yokoyama T, et al. 2011. Int Immunol. PubMed
  10. Rusert P, et al. 2011. J. Exp Med. 208:1419. PubMed
  11. Bollig N, et al. 2012. PNAS. 109:8664. PubMed
  12. Nguyen V, et al. 2012. J. Immunol. 189:1081. PubMed
  13. Jager NA, et al. 2012. J Nucl Med. 53:1229. PubMed
  14. Liberman AC, et al. 2012. Mol Immunol. 50:220. PubMed
  15. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  16. Ludin A, et al.2012. Nat Immunol. 13:1072. PubMed
  17. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  18. Tani-Ichi S, et al. 2012. PNAS. 110:612. PubMed
  19. Jin L, et al. 2013. PNAS. 110:3907. PubMed
  20. Ota T, et al. 2013. J. Immunol. 191:3179. PubMed
  21. Fu X, et al. 2013. PNAS. 110:17982. PubMed
  22. Ryan SO, et al. 2014. Glycobiology. 24:362. PubMed
  23. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  24. Pilling D, et al. 2014. PLoS One. 9:93730. PubMed
  25. Matthews JA, et al. 2014. PLoS One. 9:97707. PubMed
  26. Owen KA, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  27. Duek A, et al. 2014. Blood. 123:3943. PubMed
  28. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  29. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  30. Lundberg P, et al. 2014. J Exp Med. PubMed
  31. Martin RK, et al. 2014. PLoS One. 9:110609. PubMed
  32. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  33. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  34. Jager NA, et al. 2014. J Nucl Med. 55:1945. PubMed
  35. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  36. Cabrera-Perez J, et al. 2015. J Immunol. 194:1609. PubMed
  37. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  38. Kim Yu, et al. 2015. PLoS One. 10:120294. PubMed
  39. Zhang L, et al. 2015. J Immunol. 194:5243. PubMed
Product Citations
  1. Lebert-Ghali C, et al. 2017. Exp Hematol. 10.1016/j.exphem.2017.03.003. PubMed
  2. Audemard-Verger A, et al. 2017. J Immunol. 10.4049/jimmunol.1700430. PubMed
  3. Bradley CP et al. 2017. Cell host & microbe. 22(5):697-704 . PubMed
  4. Proietti M, et al. 2019. Nat Commun. 10:250. PubMed
  5. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  6. Pedersen NW, et al. 2019. Nat Commun. 10:837. PubMed
  7. Hermann BP, et al. 2018. Cell Rep. 25:1650. PubMed
  8. Misumi I et al. 2019. Cell Rep. 27(2):514-524 . PubMed
  9. Misumi I et al. 2019. Cell Rep. 27(5):1387-1396 . PubMed
  10. Schoeler K, et al. 2019. FEBS J. 10.1111/febs.14934. PubMed
  11. Nagatake T, et al. 2019. Mucosal Immunol. 12:p1082. PubMed
  12. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
  13. Perruzza L, et al. 2019. Sci Rep. 9:9315. PubMed
  14. Schneider C, et al. 2018. Cell. 174:271. PubMed
  15. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  16. Van Gool F, et al. 2019. Immunity. 50:362. PubMed
  17. Cohen–Khait R, et al. 2017. Structure. 25:1867. PubMed
  18. Guichard V, et al. 2017. Elife. 6:e27215. PubMed
  19. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  20. Wang L, et al. 2019. Cell Rep. 29:1848. PubMed
  21. Viborg N, et al. 2019. Oncoimmunology. 8:e1663107. PubMed
  22. Hurrell BP, et al. 2019. Cell Rep. 29:4509. PubMed
  23. Delacher M, et al. 2020. Immunity. 52:295. PubMed
  24. Pazina T, et al. 2019. Cancer Immunol Res. 7:1633. PubMed
  25. Catapano M, et al. 2020. J Invest Dermatol. 140:816. PubMed
  26. Tashita C, et al. 2020. World J Gastroenterol. 26:918. PubMed
  27. Hansen UK, et al. 2020. Front Immunol. 11:373. PubMed
  28. Kimura T, et al. 2019. Am J Physiol Lung Cell Mol Physiol. 317:L271. PubMed
  29. Chou W, et al. 2006. Blood. 108:3005. PubMed
  30. Kroeger K, et al. 2009. J Leukoc Biol. 86:769. PubMed
  31. Malagolini N, et al. 2009. Glycobiology. 0.911111111. PubMed
  32. King I, et al. 2010. J Immunol. 185:6138. PubMed
  33. Yi Y, et al. 2010. J Biol Chem. 285:21233. PubMed
  34. Rusert P, et al. 2011. J Exp Med. 208:1419. PubMed
  35. Yokoyama T, et al. 2011. J Immunol. 208:885. PubMed
  36. Jiang H, et al. 2011. PLoS One. 6:e27464. PubMed
  37. Bollig N, et al. 2012. Proc Natl Acad Sci U S A. 109:8664. PubMed
  38. GR N, et al. 2012. PLoS One. 7:e39045. PubMed
  39. Nguyen V, et al. 2012. J Immunol. 189: 1081. PubMed
  40. Jager N, et al. 2012. J Nucl Med. 53:1222. PubMed
  41. Liberman A, et al. 2012. Mol Immunol. 50:220. PubMed
  42. Ogawa H, et al. 2012. Biochem Biophys Res Commun. 420:114. PubMed
  43. Ludin A, et al. 2012. Nat Immunol. 13:1072. PubMed
  44. Sugiura D, et al. 2012. PLoS One. 7:e44770. PubMed
  45. Tani-ichi S, et al. 2012. Proc Natl Acad Sci U S A. 110:612. PubMed
  46. Jin L, et al. 2013. Proc Natl Acad Sci U S A. 110:3907. PubMed
  47. Ota T, et al. 2013. J Immunol. 191:3179. PubMed
  48. Fu X, et al. 2013. Proc Natl Acad Sci U S A. 110:17892. PubMed
  49. Ryan S, et al. 2014. Glycobiology. 24:262. PubMed
  50. Lyngaa R, et al. 2014. Clin Cancer Res. 20:1768. PubMed
  51. Pilling D, Gomer R 2014. PLoS One. 9:93730. PubMed
  52. Mathews J, et al. 2014. PLoS One. 9:97707. PubMed
  53. Owen K, et al. 2014. PLoS Pathog. 10:1004159. PubMed
  54. Duek A, et al. 2014. Blood. 123:3943. PubMed
  55. Madireddi S, et al. 2014. J Exp Med. 211:1433. PubMed
  56. Skogberg G, et al. 2014. J Immunol. 193:2187. PubMed
  57. Lundberg P, et al. 2014. J Exp Med. 211:2213. PubMed
  58. Martin R, et al. 2014. PLoS One. 9:110609. PubMed
  59. Rai S, et al. 2014. PLoS One. 9:109441. PubMed
  60. Santoyo-Ramos P, et al. 2014. PLoS One. 9:112580. PubMed
  61. Jager N, et al. 2014. J Nucl Med. 55:1945. PubMed
  62. Kong S, et al. 2014. J Immunol. 193:5515. PubMed
  63. Cabrera-Perez C, et al. 2015. J Immunol . 194:1609-20. PubMed
  64. Lucas A, et al. 2015. PLoS One. 10:117160. PubMed
  65. Kim Y, et al. 2015. PLoS One. 10:120294. PubMed
  66. Zhang L, et al. 2015. J Immunol. 194:5423. PubMed
  67. Suzuki M, et al. 2015. J Immunol. 195: 1273-1281. PubMed
  68. Gaudreault V, et al. 2015. PLoS One. 10: e0140805. PubMed
  69. Mainini F, et al. 2015. PLoS One. 10: 0145403. PubMed
  70. Jin L, et al. 2016. Stem Cell Res. 16:40-53. PubMed
  71. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  72. Tagawa M, et al. 2016. PLoS One. 11: 0150030. PubMed
  73. Tripathi S, et al. 2016. Proc Natl Acad Sci U S A. 113: 1555 - 1564. PubMed
  74. Calés C, et al. 2016. Mol Cell Biol. 36: 900 - 912. PubMed
  75. Wan X, Thomas J, Unanue E 2016. J Exp Med. 213: 967 - 978. PubMed
  76. Spanier J, et al. 2016. Nat Commun. 7:11804. PubMed
  77. J L, et al. 2016. Sci Rep. 6:30904. PubMed
  78. Morita N, et al. 2016. Innate Immunity. 10.1177/1753425916672312. PubMed
  79. Jennings R, et al. 2016. Int Immunol. 28: 503 - 512. PubMed
  80. Barcala Tabarrozzi A, et al. 2016. Sci Rep. 6:36646. PubMed
  81. Hauser J, et al. 2016. Mol Immunol. 80:78-90. PubMed
  82. Han X, et al. 2017. Mol Ther. 25(1):274-284. PubMed
  83. Locke F, et al. 2017. Mol Ther. 25(1):285-295. PubMed
  84. Mazor Y, et al. 2017. Sci Rep. 7:40098. PubMed
  85. Wang W, et al. 2020. STAR Protoc. 1:100218. PubMed
  86. James KR, et al. 2020. Nat Immunol. 1.113194444. PubMed
  87. Dyson MR, et al. 2020. MAbs. 12:1829335. PubMed
  88. Mizoguchi I, et al. 2020. J Clin Invest. 130:6124. PubMed
  89. Yamanaka S, et al. 2021. STAR Protocols. 2(1):100314. PubMed

Related FAQs

There are no FAQs for this product.
Go To Top Version: 4    Revision Date: 06/18/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

ProductsHere
Insert Note Here
Save Close Clear
Lab Timer
Tools
Login/Register
Remember me
Forgot your password? Reset Password
Request an Account