APC/Fire™ 750 anti-mouse CD34 Antibody

Pricing & Availability
Clone
HM34 (See other available formats)
Other Names
Mucosialin
Isotype
Armenian Hamster IgG
Ave. Rating
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Product Citations
publications
HM34_APCFire750_CD34_Antibody_080417
Mouse fibroblast NIH/3T3 cells were stained with CD34 (Clone HM34) APC/Fire™ 750 (filled histogram) or Armenian hamster IgG APC/Fire™ 750 isotype control (open histogram).
  • HM34_APCFire750_CD34_Antibody_080417
    Mouse fibroblast NIH/3T3 cells were stained with CD34 (Clone HM34) APC/Fire™ 750 (filled histogram) or Armenian hamster IgG APC/Fire™ 750 isotype control (open histogram).
See APC/Fire™ 750 spectral data
Cat # Size Price Quantity Avail. Save
128613 25 µg $135
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128614 100 µg $310
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Description

CD34 is a highly glycosylated hematopoietic progenitor antigen.Two isoforms of CD34 have been reported. CD34 is expressed on hematopoietic progenitors, as well as endothelial cells, brain and testis. CD34 is thought to function as an adhesion molecule for attachment of stem cells to extracellular matrix or stromal cells.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Mouse CD34 transfected BHK cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions. The solution is free of unconjugated APC/Fire™ 750 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.

Excitation Laser
Red Laser (633 nm)
Application Notes

The HM34 antibody does not stain bone marrow cells like some other mouse CD34 antibodies, probably because the antibody recognizes a different epitope from other mAbs.

Product Citations
  1. Mann M, et al. 2018. Cell Rep. 25:2992. PubMed
Publication Library
RRID
AB_2715984 (BioLegend Cat. No. 128613)
AB_2715985 (BioLegend Cat. No. 128614)

Antigen Details

Structure
Type I membrane protein, highly glycosylated, 75-120 kd, two isoforms reported.
Distribution

Hematopoietic progenitors, brain, testis, endothelial cells

Function
Possible adhesion molecules thought to function in early hematopoiesis by mediating attachment of stem cells to bone marrow extracellular matrix or stromal cells. Present carbohydrate ligands to selectins.
Ligand/Receptor
L-selectin, and others.
Cell Type
Endothelial cells, Hematopoietic stem and progenitors
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Garlanda C, et al. 1997. Eur J Cell Biol 73:368
2. Brown J, et al. 1991. Int Immunol 3:175

Gene ID
12490 View all products for this Gene ID
UniProt
View information about CD34 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 0    Revision Date: 08/04/2017

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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