Human peripheral blood lymphocytes were stained with CD16 PE and CD56 (clone 5.1H11) APC/Fire™ 750 (top) or mouse IgG1, κ APC/Fire™ 750 isotype control (bottom).
Human peripheral blood lymphocytes were stained with CD16 PE and CD56 (clone 5.1H11) APC/Fire™ 750 (top) or mouse IgG1, κ APC/Fire™ 750 isotype control (bottom).
CD56 is a single transmembrane glycoprotein also known as NCAM (neural cell adhesion molecule), Leu-19, or NKH1. It is a member of the Ig superfamily. The 140 kD isoform is expressed on NK and NKT cells. CD56 is also expressed in the brain (cerebellum and cortex) and at neuromuscular junctions. Certain large granular lymphocyte (LGL) leukemias, small-cell lung carcinomas, neuronal derived tumors, myelomas, and myeloid leukemias also express CD56. CD56 plays a role in homophilic and heterophilic adhesion via binding to itself or heparan sulfate.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 787 nm.
Application References
(PubMed link indicates BioLegend citation)
Walsh FS, et al. 1981. Nature 289:60. (FC)
Pavlath GK, et al. 1986. J. Cell Biol. 102:124. (FC)
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