APC/Fire 750 anti-human CD279 PD-1 Antibody anti-CD279

Pricing & Availability

Clone: EH12.2H7 (See other available formats)
Regulatory Status: RUO
Other Names: PD-1, PDCD1
Isotype: Mouse IgG1, κ
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Product Citations: publications

EH12point2H7_APCFire750_CD279_Antibody_042116 — PHA-stimulated (day 3) human peripheral blood lymphocytes were stained with CD279 (clone EH12.2H7) APC/Fire™ 750 (filled histogram) or mouse IgG1, κ APC/Fire™ 750 isotype control (open histogram).

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329953

25 tests

$176

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329954

100 tests

$382

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Description

Programmed cell death 1 (PD-1), also known as CD279, is a 55 kD member of the immunoglobulin superfamily. CD279 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) in the cytoplasmic region and plays a key role in peripheral tolerance and autoimmune disease. CD279 is expressed predominantly on activated T cells, B cells, and myeloid cells. PD-L1 (B7-H1) and PD-L2 (B7-DC) are ligands of CD279 (PD-1) and are members of the B7 gene family. Evidence suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Interaction of CD279 ligands results in inhibition of T cell proliferation and cytokine secretion.

Product Details

Technical data sheet

Product Details

Verified Reactivity

Human

Reported Reactivity

African Green, Baboon, Chimpanzee, Common Marmoset, Cynomolgus, Rhesus, Squirrel Monkey

Antibody Type

Monoclonal

Host Species

Mouse

Formulation

Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)

Preparation

The antibody was purified by affinity chromatography and conjugated with APC/Fire™ 750 under optimal conditions.

Concentration

Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)

Storage & Handling

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood.

* APC/Fire™ 750 has a maximum excitation of 650 nm and a maximum emission of 774 nm.

Application Notes

Additional reported applications (for the relevant formats) include: blocking of ligand binding^1-3, immunohistochemical staining of paraformaldehyde fixed frozen sections¹³, and spatial biology (IBEX)^15,16. The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 329911 and 329912). For highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 329926) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References (PubMed link indicates BioLegend citation)

Dorfman DM, et al. 2006 Am. J. Surg. Pathol. 30:802. (FA)
Radziewicz H, et al. 2007. J. Virol. 81:2545. (FA)
Velu V, et al. 2007. J. Virol. 81:5819. (FA)
Zahn RC, et al. 2008. J. Virol. 82:11577. PubMed
Chang WS, et al. 2008. J. Immunol. 181:6707. (FC) PubMed
Nakamoto N, et al. 2009. PLoS Pathog. 5:e1000313. (FA)
Jones RB, et al. 2009. J. Virol. 83:8722. (FC) PubMed
Vojnov L, et al. 2010. J. Virol. 84:753. (FC) PubMed
Radziewicz H, et al. 2010. J. Immunol. 184:2410. (FC) PubMed
Monteriro P, et al. 2011. J. Immunol. 186:4618. PubMed
Conrad J, et al. 2011. J. Immunol. 186:6871. PubMed
Salisch NC, et al. 2010. J. Immunol. 184:476. (Rhesus reactivity)
Li H and Pauza CD. 2015. Eur. J. Immunol. 45:298. (IHC)
Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Radtke AJ, et al. 2020. Proc Natl Acad Sci USA. 117:33455-33465. (SB) PubMed
Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed

Product Citations

Forconi CS, et al. 2020. Frontiers in Cellular and Infection Microbiology. 0.529166667. PubMed
Greenberg J, et al. 2022. JCI Insight. Online ahead of print. PubMed
Acharya N, et al. 2020. Immunity. 53(3):658-671.e6. PubMed
Ferry GM, et al. 2022. Front Immunol. 13:863155. PubMed
Hunegnaw R, et al. 2019. Front Immunol. 1.484027778. PubMed
Darcis G, et al. 2020. Cell Rep. 30:2284. PubMed

RRID

AB_2616720 (BioLegend Cat. No. 329953)
AB_2616721 (BioLegend Cat. No. 329954)

Antigen Details

Structure: Immunoglobulin superfamily
Distribution: Transiently expressed on CD4^- CD8^- thymocytes; upregulated in thymocytes and splenic T and B lymphocytes; expressed on activated myeloid cells
Ligand/Receptor: B7-H1 (also known as PD-L1) and B7-DC (PD-L2)
Cell Type: B cells, Lymphocytes, T cells, Thymocytes, Tregs
Biology Area: Cancer Biomarkers, Immunology, Inhibitory Molecules
Molecular Family: CD Molecules, Immune Checkpoint Receptors
Gene ID: 5133 View all products for this Gene ID
UniProt: View information about CD279 on UniProt.org

Documentation

Reviews

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Related Protocols

Cell Surface Flow Cytometry Staining Protocol

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Description	Clone	Applications

Related Pages & Pathways

Pathways

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Other Formats

View All CD279 Reagents Request Custom Conjugation

Description	Clone	Applications
Brilliant Violet 421™ anti-human CD279 (PD-1)	EH12.2H7	FC
Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
FITC anti-human CD279 (PD-1)	EH12.2H7	FC
PE anti-human CD279 (PD-1)	EH12.2H7	FC,SB
APC anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 647 anti-human CD279 (PD-1)	EH12.2H7	FC
PerCP/Cyanine5.5 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Pacific Blue™ anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Cyanine7 anti-human CD279 (PD-1)	EH12.2H7	FC
Purified anti-human CD279 (PD-1) (Maxpar® Ready)	EH12.2H7	FC,CyTOF®
Brilliant Violet 605™ anti-human CD279 (PD-1)	EH12.2H7	FC
Ultra-LEAF™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC-F
Brilliant Violet 711™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 785™ anti-human CD279 (PD-1)	EH12.2H7	FC
Brilliant Violet 510™ anti-human CD279 (PD-1)	EH12.2H7	FC
Biotin anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Dazzle™ 594 anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 488 anti-human CD279 (PD-1)	EH12.2H7	FC
PerCP anti-human CD279 (PD-1)	EH12.2H7	FC
GoInVivo™ Purified anti-human CD279 (PD-1)	EH12.2H7	FC,Block,IHC
Brilliant Violet 650™ anti-human CD279 (PD-1)	EH12.2H7	FC
Alexa Fluor® 700 anti-human CD279 (PD-1)	EH12.2H7	FC
APC/Fire™ 750 anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-A0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-B0088 anti-human CD279 (PD-1)	EH12.2H7	PG
TotalSeq™-C0088 anti-human CD279 (PD-1)	EH12.2H7	PG
Brilliant Violet 750™ anti-human CD279 (PD-1)	EH12.2H7	FC
TotalSeq™-D0088 anti-human CD279 (PD-1)	EH12.2H7	PG
PE/Fire™ 640 anti-human CD279 (PD-1)	EH12.2H7	FC
PE/Cyanine5 anti-human CD279 (PD-1)	EH12.2H7	FC

Certificate of Analysis

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Go To Top Version: 1 Revision Date: 04/21/2016

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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