APC/Cyanine7 anti-rat CD4 Antibody

Pricing & Availability
W3/25 (See other available formats)
Regulatory Status
Other Names
T4, L3T4, W3/25
Mouse IgG1, κ
Ave. Rating
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Product Citations
LOU rat splenocytes stained with W3/25 APC/Cyanine7
  • W3slash25_APCCy7_022309.jpg
    LOU rat splenocytes stained with W3/25 APC/Cyanine7
Compare all formats See APC/Cyanine7 spectral data
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201517 25 µg $154
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201518 100 µg $325
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CD4 is a 55 kD glycoprotein also known as T4. Rat CD4 is a member of the immunoglobulin superfamily and is expressed on majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells). CD4 is a T cell co-receptor that interacts with the MHC class II molecule and is involved in T cell activation. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Rat thymocyte membrane glycoproteins
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The W3/25 antibody has been shown to inhibit IL-2 production by T helper cells and to prevent autoimmune T cell transfer in an MBP induced EAE model in vivo. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections1,2, inhibition of IL-2 production3, inhibition of MBP-induced T cell activation in EAE transfer model3.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
  2. Shioji K, et al. 2001. Circulation Res. 89:540. (IHC)
  3. Mannie MD, et al. 1993. J. Immunol. 151:7293.
  4. Kurtz CC, et al. 2007. Dev. Comp. Immunol. 31:415. PubMed
Product Citations
  1. Ruppert KA, et al. 2018. Sci Rep. 8:480. PubMed
  2. Pai AV, et al. 2018. Physiology (Bethesda). 33:254. PubMed
  3. Alsheikh AJ, et al. 2019. Am J Physiol Regul Integr Comp Physiol. 317:R182. PubMed
  4. Li B, et al. 2020. Cell Mol Immunol. 1.386111111. PubMed
  5. Almolda B, et al. 2011. PLoS One. 6:e27473. PubMed
  6. Xie L, et al. 2014. J Immunol. 192:6009. PubMed
  7. Fehrenbach DJ, et al. 2020. Exp Physiol. 105:864. PubMed
AB_1186088 (BioLegend Cat. No. 201517)
AB_1186084 (BioLegend Cat. No. 201518)

Antigen Details

Ig superfamily, 55 kD

Majority of thymocytes, macrophages, and a peripheral T cell subset (T helper cells)

T cell co-receptor, T cell activation
MHC class II molecule
Cell Type
Macrophages, T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules
Antigen References

1. Brideau RJ, et al. 1980. Eur. J. Immunol. 10:609.
2. Clark SJ, et al. 187. P. Natl. Acad. Sci. USA 84:1649.

Gene ID
24938 View all products for this Gene ID 24939 View all products for this Gene ID
View information about CD4 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

  • Purified anti-rat CD4

  • FITC anti-rat CD4

  • PE anti-rat CD4

  • APC anti-rat CD4

  • Alexa Fluor® 488 anti-rat CD4

  • APC/Cyanine7 anti-rat CD4

  • PE/Cyanine7 anti-rat CD4

  • PerCP/Cyanine5.5 anti-rat CD4


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