APC/Cyanine7 anti-mouse CD117 (c-kit) Antibody

Pricing & Availability
Clone
2B8 (See other available formats)
Regulatory Status
RUO
Other Names
c-KIT, Stem Cell Factor Receptor (SCFR)
Isotype
Rat IgG2b, κ
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Product Citations
publications
2B8_APCCyanine7_CD117_Antibody_022519
C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, ? APC/Cyanine7 isotype control (right).
  • 2B8_APCCyanine7_CD117_Antibody_022519
    C57BL/6 mouse bone marrow cells were stained with CD11b FITC and CD117 (c-kit) (clone 2B8) APC/Cyanine7 (left) or rat IgG2b, ? APC/Cyanine7 isotype control (right).
Compare all formats See APC/Cyanine7 spectral data
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105825 25 µg $88
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105826 100 µg $215
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Description

CD117 is a 145 kD immunoglobulin superfamily member also known as c-Kit and stem cell factor receptor (SCFR). It is a transmembrane tyrosine-kinase receptor that binds the c-Kit ligand (also known as steel factor, stem cell factor, and mast cell growth factor). CD117 is expressed on hematopoietic stem cells (including multipotent hematopoietic stem cells, progenitors committed to myeloid and/or erythroid lineages, and T and B cell precursors), mast cells, and acute myeloid leukemia (AML) cells. CD117 interaction with its ligand is critical for the development of hematopoietic stem cells.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse bone marrow mast cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is = 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, immunohistochemistry of acetone fixed frozen sections2, and spatial biology (IBEX)5,6. The 2B8 antibody does not block c-Kit activity.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Ikuta K, et al. 1992. P. Natl. Acad. Sci. USA 89:1502. (FC)
  2. Podd BS, et al. 2006. J. Immunol. 176:6532. PubMed (IHC)
  3. Bachelet I, et al. 2008. J. Immunol. 180:6064. PubMed (FC)
  4. Charles N, et al. 2010. Nat. Med. 16:701. PubMed (FC)
  5. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  6. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Reeh K, et al. 2014. Development. 141:2950. PubMed
  2. Rüder C, et al. 2014. PLoS One. 9:104644. PubMed
  3. Abdala-Valencia H, et al. 2015. J Immunol. 195: 1377-1387. PubMed
  4. Bikorimana E, et al. 2014. J Vis Exp. 92: 52022. PubMed
  5. Ku C, et al. 2015. Genes Dev. 29: 1930-1941. PubMed
  6. Wu X, et al. 2016. Nat Commun. 7:10533. PubMed
  7. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
  8. Kurkewich J, et al. 2016. PLoS One. 11: 0161468. PubMed
  9. Rossnagl S, et al. 2016. PLoS Biol. 14: 1002562. PubMed
  10. Hu X, et al. 2016. Nat Commun. 7:13095. PubMed
  11. Zysset D, et al. 2016. Nat Commun. 7:13151. PubMed
  12. Carr M, et al. 2016. Proc Natl Acad Sci U S A. 113(52):15024-15029. PubMed
  13. Kang YA, et al. 2020. J Exp Med. 217:00:00. PubMed
  14. Elahi S, et al. 2020. Stem Cell Res. 43:101710. PubMed
  15. Li Y, et al. 2021. Nat Commun. 0.843055556. PubMed
  16. Li Y, et al. 2020. Cell Stem Cell. 27(5):732-747.e7. PubMed
  17. Murakami K, et al. 2021. Cell Reports. 34(1):108579. PubMed
  18. Riether C, et al. 2021. Cell Reports. 34(4):108663. PubMed
  19. Chen R, et al. 2021. Cell Reports. 34(7):108751. PubMed
  20. Paiva RA, et al. 2021. Cell Reports. 35(2):108967. PubMed
  21. Young K, et al. 2021. Cell Stem Cell. . PubMed
  22. Iturri L, et al. 2021. Immunity. . PubMed
  23. Mana MD, et al. 2021. Cell Reports. 35(10):109212. PubMed
  24. Duarte D, et al. 2018. Cell Stem Cell. 22:64. PubMed
  25. Comoglio F, et al. 2018. Genome Res. 10.1101/gr.227272.117. PubMed
  26. Sevin M, et al. 2018. Nat Commun. 9:1431. PubMed
  27. Nakamura‐Ishizu A et al. 2018. Cell reports. 25(7):1772-1785 . PubMed
  28. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  29. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
  30. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  31. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  32. Chaudhury S, et al. 2018. Nat Commun. 9:5280. PubMed
  33. Khoramian Tusi B, et al. 2018. Exp Hematol. 60:21. PubMed
  34. Li X, et al. 2019. Stem Cells. 37:937. PubMed
  35. Tan DQ, et al. 2019. Cell Rep. 26:2316. PubMed
  36. Minuesa G, et al. 2019. Nat Commun. 10:2691. PubMed
  37. Bell CC, et al. 2019. Nat Commun. 10:2723. PubMed
  38. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  39. Clemente–Casares X, et al. 2017. Immunity. 47:974. PubMed
  40. Kobayashi H, et al. 2020. Cell Reports. 28(1):145-158.e9.. PubMed
  41. Ito K et al. 2019. Cell Rep. 28(10):2480-2490 . PubMed
  42. Shigeta A, et al. 2019. Dev Cell. 48:617. PubMed
  43. Yu VWC, et al. 2017. Cell. 168:944. PubMed
  44. Yu VWC et al. 2016. Cell. 167(5):1310-1322 . PubMed
  45. Kassas–Guediri A, et al. 2017. PLoS One. 12:e0173815. PubMed
  46. Nurkhametova D, et al. 2019. Front Cell Neurosci. 13:45. PubMed
  47. Nowlan B, et al. 2019. Haematologica. 105:71. PubMed
  48. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  49. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  50. Domingues AF, et al. 2020. Elife. 9:e51754. PubMed
  51. Fransén Pettersson N, et al. 2018. PLoS One. 13:e0203228. PubMed
  52. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  53. Woods B, et al. 2019. Clin Cancer Res. 25:5901. PubMed
  54. Krivtsov AV, et al. 2020. Cancer Cell. 36(6):660-673.e11.. PubMed
  55. Ramos CV, et al. 2020. Cell Reports. 32(3):107910. PubMed
  56. Viny AD, et al. 2019. Cell Stem Cell. 25:682. PubMed
  57. Nagai M, et al. 2020. Cell. 178(5):1072-1087.e14.. PubMed
  58. Stine RR, et al. 2020. Cell Stem Cell. 25(6):830-845. PubMed
  59. Kobayashi H, et al. 2020. STAR Protoc. 1:100078. PubMed
  60. Taylor D, et al. 2011. J Immunol. 186:5956. PubMed
RRID
AB_1626280 (BioLegend Cat. No. 105825)
AB_1626278 (BioLegend Cat. No. 105826)

Antigen Details

Structure
Ig superfamily, 145 kD
Distribution

Hematopoietic stem cells, AML, mast cells

Function
Growth factor receptor, tyrosine kinase
Ligand/Receptor
Stem Cell Factor (SCF)
Cell Type
Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
Biology Area
Immunology, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Galli SJ, et al. 1994. Adv. Immunol. 55:1.
3. Ikuta K, et al. 1992. Annu. Rev. Immunol. 10:759.
4. Besmer P, et al. 1986. Nature 320:415.
5. Witte ON. 1990. Cell 63:5.

Gene ID
16590 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 02/25/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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