APC/Cyanine7 anti-human CD4 Antibody

Pricing & Availability
Clone
A161A1 (See other available formats)
Other Names
T4/Leu-3
Isotype
Rat IgG2b, κ
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Product Citations
publications
A161A1_APCCyanine7_CD4_Antibody_020119_updated.png
Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram).
  • A161A1_APCCyanine7_CD4_Antibody_020119_updated.png
    Human peripheral blood lymphocytes were stained with CD4 (clone A161A1) APC/Cyanine7 (filled histogram) or Rat IgG2b, ? APC/Cyanine7 isotype control (open histogram).
See APC/Cyanine7 spectral data
Cat # Size Price Quantity Avail. Save
357415 25 tests $105
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357416 100 tests $265
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Description

CD4, also known as T4/Leu-3, is a 55 kD single-chain type I transmembrane glycoprotein and member of the immunoglobulin superfamily. It is expressed on most thymocytes, helper T cells, type II NKT cells, and monocytes/macrophages. CD4 is part of the TCR/CD3 complex, binds to β2 domain from the MHC class II molecule, and participates in TCR signal transduction. CD4 is the receptor of IL-16 and is a coreceptor for the human immunodeficiency virus (HIV) and human herpes virus 7 (HHV-7).

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Human CD4 T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cyanine7 under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Product Citations
  1. Peng S et al. 2019. Cell Rep. 28(10):2728-2738 . PubMed
  2. Wu J et al. 2019. Immunity. 50(5):1218-1231 . PubMed
RRID
AB_2616809 (BioLegend Cat. No. 357415)
AB_2616810 (BioLegend Cat. No. 357416)

Antigen Details

Structure
55 kD, type I transmembrane glycoprotein, member of the Ig superfamily
Distribution

Helper T cells, subset of thymocytes, monocytes/macrophages, type II NKT cells

Interaction
Part of the TCR/CD3 complex
Ligand/Receptor
β2 domain from the MHC class II
Cell Type
Macrophages, Monocytes, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Zhu J, et al. 2010. Annu Rev. Immunol. 28:445.
2. Vignali DA. 2010. J. Immunol. 184:5933.
3. Zhou L, et al. 2009. Immunity 30:646.
4. Singer A, et al. 2008. Nat. Rev. Immunol. 8:788.
5. Zhu J and Paul WE. 2008. Blood 112:1557.

Gene ID
920 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 2    Revision Date: 02/01/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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