APC/Cy7 anti-mouse Ly-6G Antibody

Pricing & Availability
Clone
1A8 (See other available formats)
Other Names
Lymphocyte antigen 6 complex, locus G
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
1A8_APCCy7_072210
C57BL/6 bone marrow cells stained with 1A8 APC/Cy7
  • 1A8_APCCy7_072210
    C57BL/6 bone marrow cells stained with 1A8 APC/Cy7
See APC/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
127623 25 µg $90
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127624 100 µg $275
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Description

Lymphocyte antigen 6 complex, locus G (Ly-6G), a 21-25 kD GPI-anchored protein, is expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Ly-6G transfected EL-4J cell line.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

While 1A8 recognizes only Ly-6G, clone RB6-8C5 recognizes both Ly-6G and Ly-6C. Clone RB6-8C5 binds with high affinity to mouse Ly-6G molecules and to a lower extent to Ly-6C15. Clone RB6-8C5 impairs the binding of anti-mouse Ly-6G clone 1A815. However, clone RB6-8C5 is able to stain in the presence of anti-mouse Ly-6C clone HK1.416.

Additional reported applications (for the relevant formats) include: immunohistochemistry9 of frozen sections10 and paraffin-embedded sections11, and depletion4, 12-14. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 127620). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127632) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Fleming TJ, et al. 1993. J. Immunol. 151:2399. (FC)
  2. Daley JM, et al. 2008. J. Leukocyte Biol. 83:1. (FC)
  3. Dietlin TA, et al. 2007. J. Leukocyte Biol. 81:1205. (FC)
  4. Daley J, et al. 2007. J. Leukocyte Biol. doi:10.1189. (Deplete) PubMed
  5. Tadagavadi RK, et al. 2010. J. Immunol. 185:4904. PubMed
  6. Sumagin R, et al. 2010. J. Immunol. 185:7057. PubMed
  7. Guiducci C, et al. 2010. J. Exp Med. 207:2931. PubMed
  8. Fujita M, et al. 2011. Cancer Res. 71:2664. PubMed
  9. Van Leeuwen, et al. 2008. Arterioscler. Thromb. Vasc. Biol. 28:84. (IHC)
  10. Kowanetz M, et al. 2010. P. Natl. Acad. Sci. USA 107:21248. [supplementary data] (IHC)
  11. Esbona K, et al. 2016. Breast Cancer Res. 18:35. (IHC)
  12. Wojtasiak M, et al. 2010. J. Gen. Virol. 91:2158. (FC, Deplete)
  13. Jaeger BN, et al. 2012. J. Exp. Med. 209:565. (Deplete)
  14. Wozniak KL, et al. 2012. BMC Immunol. 13:65 (FC, Deplete)
  15. Ribechini E, et al. 2009. Eur. J. Immunol. 39:3538.
  16. Ng LG, et al. 2011. J Invest. Dermatol. 131:2058. PubMed
  17. Ma C, et al. 2012. J. Leukoc. Biol. 92:1199.
  18. McCartney-Francis, N, et al. 2014. J Leukoc. Biol. 96:917. PubMed
  19. Her Z, et al. 2014. EMBO Mol. Med. 7:24. PubMed
Product Citations
  1. Price P, et al. 2014. J Virol. 88:10840. PubMed
  2. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
  3. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  4. Ellis G, et al. 2015. EMBO Rep. 16: 1203-1218. PubMed
  5. Fontana MF, et al. 2015. Parasite Immunol. 37: 470-478. PubMed
  6. Hilliard K, et al. 2015. Infect Immun . 83: 4015 - 4027. PubMed
  7. Zhang J, et al. 2015. Sci Rep. 5: 15553. PubMed
  8. Tan X, et al. 2015. Sci Rep. 5: 15702. PubMed
  9. Däbritz J, et al. 2016. Sci Rep. 6:20584. PubMed
  10. Woods T, et al. 2016. J Virol. 90: 2783 - 2793. PubMed
  11. Tan X, et al. 2016. PLoS One. 11: 0160985. PubMed
  12. Saha S, et al. 2016. Nat Commun. 7:13096. PubMed
  13. Sen D, et al. 2016. PLoS One. 11:e0165064. PubMed
  14. Wilson R, et al. 2016. Nat Commun. 7:13597. PubMed
  15. Cohen M 2018. Cell. 175:1031. PubMed
  16. Medler TR 2018. Cancer cell. 34:561. PubMed
  17. Van Winkle JA 2018. Cell host & microbe. 24:665. PubMed
  18. Yu X, et al. 2019. Nat Commun. 10:574. PubMed
  19. Tran L, et al. 2017. Cancer Immunol Res. 5:1141. PubMed
  20. Laban H, et al. 2018. J Cell Biol. 217:1503. PubMed
  21. Minutti CM, et al. 2019. Immunity. 50:645. PubMed
  22. Hastings AK, et al. 2019. iScience. 13:339. PubMed
  23. Han Y, et al. 2019. Cell Rep. 27:835. PubMed
  24. Kubli SP, et al. 2019. Nat Commun. 10:2678. PubMed
  25. Zhang F, et al. 2019. Nat Commun. 10:3974. PubMed
Publication Library
RRID
AB_10645331 (BioLegend Cat. No. 127623)
AB_10640819 (BioLegend Cat. No. 127624)

Antigen Details

Structure
A 21-35 kD GPI-anchorded membrane protein
Distribution

Expressed on the majority of myeloid cells in bone marrow and peripheral granulocytes. The monoclonal antibody RB6-8C5 recognizes both Ly-6G and Ly-6C.

Cell Type
Granulocytes, Macrophages, Monocytes
Biology Area
Immunology, Innate Immunity
Antigen References

Fleming TJ, et al. 1993. J. Immunol. 151:2399.

Gene ID
546644 View all products for this Gene ID
UniProt
View information about Ly-6G on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 3    Revision Date: 01/06/2017

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