APC/Cy7 anti-mouse/human CD45R/B220 Antibody

Pricing & Availability
Clone
RA3-6B2 (See other available formats)
Other Names
B220
Isotype
Rat IgG2a, κ
Ave. Rating
4 reviews
Product Citations
publications
RA3-6B2_APCCy7_060308
C57BL/6 splenocytes stained with RA3-6B2 APC/Cy7
  • RA3-6B2_APCCy7_060308
    C57BL/6 splenocytes stained with RA3-6B2 APC/Cy7
See APC/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
103223 25 µg $80
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103224 100 µg $180
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Description

CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Human, Cross-Reactivity: Cat (Feline)
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone RA3-6B2 has been described to react with an epitope on the extracellular domain of the transmembrane CD45 glycoprotein which is dependent upon the expression of exon A and specific carbohydrate residues. Additional reported applications (for the relevant formats) include: immunoprecipitation1, in vitro and in vivo modulation of B cell responses2-4, and immunohistochemistry of acetone-fixed frozen sections and formalin-fixed paraffin-embedded sections5,6. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103216).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Coffman RL. 1982. Immunol. Rev. 69:5. (IP)
  2. George A, et al. 1994. J. Immunol. 152:1014. (Activ)
  3. Asensi V, et al. 1989. Immunology 68:204. (Activ)
  4. Domiati-Saad R, et al. 1993. J. Immunol. 151:5936. (Activ)
  5. Hata H, et al. 2004. J. Clin. Invest. 114:582. (IHC)
  6. Monteith CE, et al. 1996. Can. J. Vet. Res. 60:193. (IHC)
  7. Shih FF, et al. 2006. J. Immunol. 176:3438. (FC)
  8. Chang C L-T, et al. 2007. J. Immunol. 178:6984.
  9. Fazilleau N, et al. 2007. Nature Immunol. 8:753.
  10. Lang GL, et al. 2008. Blood 111:2158. PubMed
  11. Charles N, et al. 2010. Nat. Med. 16:701. (FC) PubMed
  12. del Rio ML, et al. 2011. Transpl. Int. 24:501. (FC) PubMed
  13. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
Product Citations
  1. Thayer T, et al. 2011. Diabetes. 60:2144. PubMed
  2. Sato-Hashimoto M, et al. 2011. J Immunol. 187:291. PubMed
  3. D'Auria K, et al. 2013. Infect Immun . 81:3814. PubMed
  4. Altin J, et al. 2014. Proc Natl Acad Sci U S A. 111:2067. PubMed
  5. Gazit R, et al. 2014. J Exp Med. 211:1315. PubMed
  6. Chuang H, et al. 2014. Nat Commun. 5:4602. PubMed
  7. Gough D, et al. 2014. Blood. 124:2252. PubMed
  8. Dema B, et al. 2014. J Exp Med. 211:2159. PubMed
  9. Huang Y, et al. 2015. Proc Natl Acad Sci U S A. 112:39. PubMed
  10. Chung Y, et al. 2014. J Vis Exp. 89: 51660. PubMed
  11. Fournier M, Bijl C 2015. Stem Cells Dev. 24: 2413-2422. PubMed
  12. RL M, et al. 2015. Proc Natl Acad Sci U S A. 112:6506-6514. PubMed
  13. Rodda L, et al. 2015. J Immunol. 195: 4781 - 4791. PubMed
  14. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
  15. Muppidi J, et al. 2015. J Exp Med. 212: 2213 - 2222. PubMed
  16. Huang Y, et al. 2016. J Immunol. 196: 217 - 231. PubMed
  17. Montes de Oca M, et al. 2016. PLoS Pathog. 12: 1005398. PubMed
  18. Palm A, et al. 2016. Sci Rep. 10.1038/srep20531. PubMed
  19. Andresen L, et al. 2016. PLoS One. 11: 0148428. PubMed
  20. Jacoby E, et al. 2016. Blood. 127: 1361 - 1370. PubMed
  21. Georgoudaki A, et al. 2016. Cell Rep. 15: 2000-2011. PubMed
  22. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  23. Jacoby E, et al. 2016. Nat Commun. 7:12320. PubMed
  24. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  25. Ishikura S, et al. 2016. J Biol Chem. 291: 15282 - 15291. PubMed
  26. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  27. Sochalska M, et al. 2016. Oncogene. 10.1038/onc.2016.362. PubMed
  28. BJ L, et al. 2017. J Exp Med . 10.1084/jem.20161461. PubMed
  29. Yan-Feng L, et al. 2017. Sci Rep. 7:40133. PubMed
  30. Furuta Y, et al. 2017. PLoS One. 12(2):e0172509. PubMed
  31. Petursdottir D, et al. 2017. Front Immunol. . 10.3389/fimmu.2017.01699. PubMed
  32. Uribe-Herranz M, et al. 2018. JCI Insight. 3. PubMed
  33. Kim SK, et al. 2018. Genes Dev. 32:849. PubMed
  34. Bowers E, et al. 2018. Nat Med. 24:95. PubMed
  35. Kleppe M 2018. Cancer cell. 33:29. PubMed
  36. Celik H 2018. Cancer cell. 34:741. PubMed
  37. Sun Y 2018. Cancer cell. 34:643. PubMed
  38. Stewart I 2018. Immunity. 49:477. PubMed
  39. Lam WY 2018. Cell reports. 24:2479. PubMed
  40. Kunimoto H, et al. 2018. Cancer Cell. 33:44. PubMed
  41. Chen X, et al. 2017. Cell Stem Cell. 21:747. PubMed
  42. Luo H, et al. 2019. Cell Rep. 26:945. PubMed
  43. Chen Z, et al. 2019. J Exp Med. 216:152. PubMed
  44. Isvoranu G, et al. 2019. Oncol Lett. 17:4197. PubMed
Publication Library
RRID
AB_313006 (BioLegend Cat. No. 103223)
AB_313007 (BioLegend Cat. No. 103224)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

B cells, T cell subset, NK cell subset

Function
Phosphatase, T and B cell activation
Ligand Receptor
Galectin-1, CD2, CD3, CD4
Cell Type
B cells, T cells, NK cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID 5788 View all products for this Gene ID
UniProt
View information about CD45R on UniProt.org

Related FAQs

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Go To Top Version: 3    Revision Date: 04/25/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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