APC/Cy7 anti-mouse CD4 Antibody

Pricing & Availability
Clone
RM4-5 (See other available formats)
Other Names
L3T4, T4
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
RM4-5_APCCy7_020608
C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC/Cy7 (filled histogram) or rat IgG2a, κ APC/Cy7 isotype control (open histogram).
  • RM4-5_APCCy7_020608
    C57BL/6 mouse splenocytes stained with CD4 (clone RM4-5) APC/Cy7 (filled histogram) or rat IgG2a, κ APC/Cy7 isotype control (open histogram).
See APC/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
100525 25 µg $80
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100526 100 µg $180
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Description

CD4 is a 55 kD protein also known as L3T4 or T4. It is a member of the Ig superfamily, primarily expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic cells. It acts as a co-receptor with the TCR during T cell activation and thymic differentiation by binding MHC class II and associating with the protein tyrosine kinase lck.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
BALB/c mouse thymocytes
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

The RM4-5 antibody blocks the binding of GK1.5 antibody and H129.19 antibody to CD4+ T cells, but not RM4-4 antibody. Additional reported applications (for the relevant formats) include: blocking of ligand binding, in vivo depletion of CD4+ cells1, and immunohistochemistry of acetone-fixed frozen tissue sections2,3,11 and paraffin-embedded sections11. Clone RM4-5 is not recommended for immunohistochemistry of formalin-fixed paraffin sections. Instead, acetone frozen or zinc-fixed paraffin sections are recommended. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100520).

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. Kruisbeek AM. 1991. In Curr. Protocols Immunol. pp. 4.1.1-4.1.5. (Block, Deplete)
  2. Nitta H, et al. 1997. Cell Vision 4:73. (IHC)
  3. Fan WY, et al. 2001. Exp. Biol. Med. 226:1045.
  4. Muraille E, et al. 2003. Infect. Immun. 71:2704. (IHC)
  5. León-Ponte M, et al. 2007. Blood 109:3139. (FC)
  6. Bourdeau A, et al. 2007. Blood doi:10.1182/blood-2006-08-044370. (FC)
  7. Matsumoto M, et al. 2007.J. Immunol.178:2499. PubMed
  8. Shigeta A, et al. 2008. Blood 112:4915. PubMed
  9. Zaborsky N, et al. 2010. J. Immunol. 184:725. PubMed
  10. Rodrigues-Manzanet R, et al. 2010. P. Natl Acad Sci USA 107:8706. PubMed
  11. Whiteland JL, et al. 1995. J. Histochem. Cytochem. 43:313. (IHC)
Product Citations
  1. Brenndörfer E, et al. 2014. J Immunol. 192:1671. PubMed
  2. Shiomi A, et al. 2014. J Immunol. 193:849. PubMed
  3. Wei M, et al. 2014. Protein Eng Des Sel. 27:289. PubMed
  4. Lu X, et al. 2015. J Immunol. 194:2011. PubMed
  5. Ulrich V, et al. 2016. EMBO Mol Med. 8: 643 - 653. PubMed
  6. Aryal B, et al. 2016. Nat Commun. 7:12313. PubMed
  7. Mencarelli A, et al. 2016. Sci Rep. 6:30802. PubMed
  8. Bankoti R, et al. 2017. Sci Rep. 10.1038/s41598-017-12171-3. PubMed
  9. Ding Z, et al. 2017. Sci Rep. 10.1038/s41598-017-12488-z. PubMed
  10. Saigusa R, et al. 2018. Arthritis Res Ther. 20:23. PubMed
  11. Cobbold SP, et al. 2018. Front Immunol. 9:1381. PubMed
  12. Jarick KJ, et al. 2018. Front Immunol. 9:1468. PubMed
  13. Biton M 2018. Cell. 175:1307. PubMed
  14. Ogawa C 2018. Cell reports. 25:19. PubMed
  15. Turner JS, et al. 2018. Cell Rep. 25:1395. PubMed
  16. Tordesillas L, et al. 2018. Nat Commun. 9:5238. PubMed
  17. Weng Q, et al. 2018. Cell Regen (Lond). 7:7. PubMed
  18. Ding L, et al. 2018. Cell Rep. 25:2972. PubMed
  19. Herndler–Brandstetter D, et al. 2018. Immunity. 48:716. PubMed
  20. Kubota S, et al. 2019. Nat Commun. 10:1653. PubMed
  21. Howie D, et al. 2019. Front Immunol. 10:1860. PubMed
Publication Library
RRID
AB_312726 (BioLegend Cat. No. 100525)
AB_312727 (BioLegend Cat. No. 100526)

Antigen Details

Structure
Ig superfamily, 55 kD
Distribution

Majority of thymocytes, T cell subset

Function
TCR co-receptor, T cell activation
Ligand/Receptor
MHC class II molecule
Cell Type
Thymocytes, T cells, Dendritic cells, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Bierer BE, et al. 1989. Annu. Rev. Immunol. 7:579.
3. Janeway CA. 1992. Annu. Rev. Immunol. 10:645.

Gene ID
12504 View all products for this Gene ID
UniProt
View information about CD4 on UniProt.org

Related FAQs

I am unable to see expression of T cell markers such as CD3 and CD4 post activation.
TCR-CD3 complexes on the T-lymphocyte surface are rapidly downregulated upon activation with peptide-MHC complex, superantigen or cross-linking with anti-TCR or anti-CD3 antibodies. PMA/Ionomycin treatment has been shown to downregulate surface CD4 expression. Receptor downregulation is a common biological phenomenon and so make sure that your stimulation treatment is not causing it in your sample type.
Go To Top Version: 3    Revision Date: 01/29/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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