APC/Cy7 anti-human CD5 Antibody

Pricing & Availability
Clone
L17F12 (See other available formats)
Workshop
II T7
Other Names
Leu-1, Ly-1, T1, Tp67, Leu1
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
L17F12_APCCy7_CD5_Antibody_FC_102714
Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) APC/Cy7 (filled histogram) or mouse IgG2a, κ APC/Cy7 isotype control (open histogram).
  • L17F12_APCCy7_CD5_Antibody_FC_102714
    Human peripheral blood lymphocytes were stained with CD5 (clone L17F12) APC/Cy7 (filled histogram) or mouse IgG2a, κ APC/Cy7 isotype control (open histogram).
See APC/Cy7 spectral data
Cat # Size Price Quantity Avail. Save
364009 25 tests $115
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364010 100 tests $275
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Description

CD5, also known as Leu-1, Ly-1 and T1, is a 67 kD single chain type I glycoprotein that is a member of the scavenger receptor superfamily. CD5 is expressed on T cells, thymocytes, B cell subsets, chronic B lymphocytic leukemia (B-Cells), and peripheral blood dendritic cells. CD5 modulates T and B cell receptor signaling, thymocyte maturation, and T-B cell interactions. One of its ligands is CD72.

Product Details
Technical data sheet

Product Details

Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human T-acute lymphoblastic leukemia (ALL) cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC/Cy7 under optimal conditions. The solution is free of unconjugated APC/Cy7 and unconjugated antibody.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats): immunoprecipitation1,3,4 and immmunohistochemical staining of frozen and formalin-fixed paraffin-embedded sections2,3,5.

Additional Product Notes
BioLegend is in the process of converting the name APC/Cy7 to APC/Cyanine7. The dye molecule remains the same, so you should expect the same quality and performance from our APC/Cyanine7 products. Please contact Technical Service if you have any questions.
Application References

(PubMed link indicates BioLegend citation)
  1. McAlister MS, et al. 1998. Protein Eng. 11:847. (IP)
  2. Butmarc JR, et al. 1998. Am. J. Clin. Pathol. 109:682. (IHC)
  3. Engleman EG, et al. 1981. Proc. Natl. Acad. Sci. USA 78:1791. (IHC, IP)
  4. Ledbetter JA, et al. 1981. J. Exp. Med. 153:310. (FC, IP)
  5. Warnke R and Levy R. 1980. J. Histochem. Cytochem. 28:771. (IHC)
Product Citations
  1. Trabanelli S, et al. 2017. Nat Commun. 10.1038/s41467-017-00678-2. PubMed
Publication Library
RRID
AB_2564505 (BioLegend Cat. No. 364009)
AB_2564506 (BioLegend Cat. No. 364010)

Antigen Details

Structure
Scavenger receptor superfamily, 67 kD
Distribution

Thymocytes, T cells, and subsets of B and dendritic cells.

Function
Modulates TCR and BCR signaling, involved in thymocyte maturation and T-B cell interaction
Ligand/Receptor
CD72
Cell Type
Thymocytes, T cells, B cells, Dendritic cells
Biology Area
Immunology, Costimulatory Molecules
Molecular Family
CD Molecules
Antigen References

1. Kipps T. 1989. Adv. Immunol. 47:117.
2. Resnick D, et al. 1994. Trends Biochem. Sci. 1:5.
3. Wood GS and Freudenthal PS. 1992. Am. J. Pathol. 141:789.

Gene ID
921 View all products for this Gene ID
UniProt
View information about CD5 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/13/2014

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*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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