APC anti-mouse/human CD15 (SSEA-1) Antibody

Pricing & Availability
Clone
MC-480 (See other available formats)
Other Names
Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1
Isotype
Mouse IgM, κ
Ave. Rating
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Product Citations
publications
MC-480_APC_CD15_Antibody_021419
Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
  • MC-480_APC_CD15_Antibody_021419
    Murine embryonic carcinoma cell line F9 was stained with CD15 (SSEA-1) (clone MC-480) APC (filled histogram) or mouse IgM APC Isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
125617 25 tests $125
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125618 100 tests $265
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Description

The MC-480 antibody reacts with mouse and human Stage-Specific Embryonic Antigen-1 (SSEA-1). It is a Lewis blood group related carbohydrate antigen, also known as X-hapten, Lewis X, 3-FAL, 3-fucosyl-N-acetyllactosamine, or CD15. The expression pattern of SSEA-1 antigen is different in humans than in mice. In mice, SSEA-1 is expressed on embryonic stem cells (ES), embryonal carcinoma cells (EC), 8-cell to blastocyst embryos, and a subset of embryonic inner cell mass. The expression on murine ES cells is decreased upon differentiation. In humans, however, SSEA-1 is not found on undifferentiated ES cells, but its expression is upregulated along with differentiation. CD15 is highly expressed on adult human granulocytes. It has been reported that SSEA-1 plays a role in cell adhesion and regulation of cell differentiation.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse, Cross-Reactivity: Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin USA).
Preparation
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
Concentration
Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup tool if you have a lot number.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1, Western blotting1, and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565. (IHC, IP, WB)
RRID
AB_2800604 (BioLegend Cat. No. 125617)
AB_2800605 (BioLegend Cat. No. 125618)

Antigen Details

Structure
Carbohydrate epitope, Lewis blood group antigens
Distribution

Murine embryonic stem cells (ES), embryonal carcinoma cells, 8-cell to blastocyst embryos; human differentiated cells, not on undifferentiated ES cells

Function
Adhesion, differentiation
Cell Type
Embryonic Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules
Antigen References

1. Solter D and Knowles BB. 1978. Proc. Natl. Acad. Sci. USA. 75:5565.
2. Harris JF, et al. 1984. J. Immunol. 132:2502.
3. Gooi HC, et al. 1981. Nature 292:156.
4. Cui L, et al. 2004. J. Histochem. Cytochem. 52:1447.

Gene ID
14345 View all products for this Gene ID 2526 View all products for this Gene ID
UniProt
View information about CD15 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/14/2019

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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