APC anti-mouse CD8b.2 Antibody

Pricing & Availability
53-5.8 (See other available formats)
Regulatory Status
Other Names
Lyt-3.2, Ly-3.2
Rat IgG1, κ
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Product Citations
C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) APC and CD8a (53-6.7) FITC
  • 53-5.8_APC_083010
    C57BL/6 mouse splenocytes stained with CD8b.2 (53-5.8) APC and CD8a (53-6.7) FITC
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140410 100 µg $232
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CD8b is the 32 kD β chain of CD8, also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8α chain on a subset of MHC class I-restricted T cells and most thymocytes. CD8 is a co-receptor for the TCR complex involved in T cell activation. The antibody 53-5.8 is specific for Ly-3.2 and has low reactivity with Ly-3.1.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Antibody Type
Host Species
Mouse thymus or spleen
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography and conjugated with APC under optimal conditions.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence3, immunohistochemical staining4 of frozen tissue section using dry ice-isopentane method and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Ledbetter J, et al. 1979. Immunol. Rev. 47:63.
  2. Ledbetter J, et al. 1980. J. Exp. Med. 152:280.
  3. Vremec D, et al. 2000. J. Immunol. 164:2978. (IF)
  4. Lawrence D, et al. 1999. J. Virol. 73:1795. (IHC)
  5. Bosselut R, et al. 1999. J. Exp. Med. 190:1517. (IP)
Product Citations
  1. Montes de Oca R, et al. 2021. Mol Cancer Ther. 20:1941. PubMed
  2. Guo L, et al. 2021. Front Immunol. 12:785457. PubMed
  3. Zhou H, et al. 2022. Oncoimmunology. 11:2057892. PubMed
  4. Michels A, et al. 2021. Mol Ther Methods Clin Dev. 23:334. PubMed
  5. Evgin L, et al. 2020. Nat Commun. 2.671527778. PubMed
  6. Evgin L, et al. 2022. Sci Transl Med. 14:eabn2231. PubMed
  7. Zhou H, et al. 2021. Cell Death Discov. 7:332. PubMed
  8. Kurashima Y, et al. 2021. Nat Commun. 12:1067. PubMed
  9. Mazumder S, et al. 2021. Heliyon. 7:e08124. PubMed
  10. Louise V Webb et al. 2019. Immunity. 50(2):348-361 . PubMed
AB_10641696 (BioLegend Cat. No. 140410)

Antigen Details

Ig superfamily, CD8β chain, 32 kD

Majority of thymocytes, cytotoxic T cell subsets

Co-receptor of TCR
MHC class I
Cell Type
T cells, Thymocytes
Biology Area
Molecular Family
CD Molecules, TCRs
Antigen References

1. Ledbetter J, et al. 1981. J. Exp. Med. 153:1503.
2. Renard V, et al. 1996. J. Exp. Med. 184:2439.

Gene ID
12526 View all products for this Gene ID
View information about CD8b.2 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.


*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.


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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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