APC anti-mouse CD45 Antibody

Pricing & Availability
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Rat IgG2b, κ
Ave. Rating
8 reviews
Product Citations
C57BL/6 mouse splenocytes stained with 30-F11 APC
  • 30-F11_APC_090507
    C57BL/6 mouse splenocytes stained with 30-F11 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
103111 25 µg $30
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103112 100 µg $85
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CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Antibody Type
Host Species
Mouse thymus or spleen
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,  immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103120).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
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  4. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  5. Wang C, et al. 2011. Hepatology. 55:108. PubMed
  6. Roberts L, et al. 2014. Infect Immun . 82:2504. PubMed
  7. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  8. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
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  12. Wang L, et al. 2015. Cancer Immunol Res. 3: 1030-1041. PubMed
  13. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
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  15. Liu L, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: 174. PubMed
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  18. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
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  22. Wong J, et al. 2016. Sci Signal. 9: ra103. PubMed
  23. Chiche A, et al. 2016. Oncogene. 10.1038/onc.2016.396. PubMed
  24. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  25. Vaas M, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16676825. PubMed
  26. Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
  27. Paiva-Oliveira E, et al. 2017. Histochem Cell Biol. 10.1007/s00418-017-1556-6. PubMed
  28. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  29. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
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  33. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
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Publication Library
AB_312976 (BioLegend Cat. No. 103111)
AB_312977 (BioLegend Cat. No. 103112)

Antigen Details

Protein tyrosine phosphatase (PTP) family, 180-240 kD

All hematopoietic cells except mature erythrocytes and platelets

Phosphatase, T and B cell activation
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
View information about CD45 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/14/2013

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