APC anti-mouse CD45 Antibody

Pricing & Availability
Clone
30-F11 (See other available formats)
Other Names
T200, Ly-5, LCA
Isotype
Rat IgG2b, κ
Ave. Rating
8 reviews
Product Citations
publications
30-F11_APC_090507
C57BL/6 mouse splenocytes stained with 30-F11 APC
  • 30-F11_APC_090507
    C57BL/6 mouse splenocytes stained with 30-F11 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
103111 25 µg $30
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103112 100 µg $85
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Description

CD45 is a 180-240 kD glycoprotein also known as the leukocyte common antigen (LCA), T200, or Ly-5. It is a member of the protein tyrosine phosphatase (PTP) family, expressed on all hematopoietic cells except mature erythrocytes and platelets. There are different isoforms of CD45 that arise from variable splicing of exons 4, 5, and 6, which encode A, B, and C determinants, respectively. CD45 plays a key role in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation state of the cell as well as cell type. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse thymus or spleen
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 30-F11 reacts with all isoforms and both CD45.1 and CD45.2 alloantigens of CD45.

Additional reported applications (for relevant formats) include: immunoprecipitation3, complement-dependent cytotoxicity1,5,  immunohistochemistry (acetone-fixed frozen sections, zinc-fixed paraffin-embedded sections and formalin-fixed paraffin-embedded sections)4,6 and Western blotting7. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 103120).

Application References

(PubMed link indicates BioLegend citation)
  1. Podd BS, et al. 2006. J. Immunol. 176:6532. (FC, CMCD) PubMed
  2. Haynes NM, et al. 2007. J. Immunol. 179:5099. (FC)
  3. Ledbetter JA, et al. 1979. Immunol. Rev. 47:63. (IP)
  4. Simon DI, et al. 2000. J. Clin. Invest. 105:293. (IHC)
  5. Seaman WE. 1983. J. Immunol. 130:1713. (CMCD)
  6. Cornet A, et al. 2001. P. Natl. Acad. Sci. USA 98:13306. (IHC)
  7. Tsuboi S and Fukuda M. 1998. J. Biol. Chem. 273:30680. (WB) PubMed
  8. Liu F, et al. 2012. Blood. 119:3295. PubMed
  9. Pelletier AN, et al. 2012. J. Immunol. 188:5561. PubMed
Product Citations
  1. Sambasivan R, et al. 2011. Development. 138:3647. PubMed
  2. Pelletier A, et al. 2012. J Immunol. 188:5561. PubMed
  3. Kang I, et al. 2012. Blood. 119:3295. PubMed
  4. Lafkas D, et al. 2013. J Cell Biol. 203:47. PubMed
  5. Wang C, et al. 2011. Hepatology. 55:108. PubMed
  6. Roberts L, et al. 2014. Infect Immun . 82:2504. PubMed
  7. Garcia-Bonilla L, et al. 2014. J Immunol. 193:2531. PubMed
  8. Hyatt L, et al. 2014. PLoS One. 9:109072. PubMed
  9. Ding X, et al. 2015. J Immunol. 194:4251. PubMed
  10. Elias S, et al. 2015. PLoS Biol. 13:1002142. PubMed
  11. Seki T, et al. 2015. J Biochem. 158: 485 - 495. PubMed
  12. Wang L, et al. 2015. Cancer Immunol Res. 3: 1030-1041. PubMed
  13. Jiang Y, et al. 2015. MBio. 6: e01426-15. PubMed
  14. Garcia-Bonilla L, et al. 2015. J Neurosci. 35: 14783 - 14793. PubMed
  15. Liu L, et al. 2015. Neurol Neuroimmunol Neuroinflamm. 2: 174. PubMed
  16. Sim S, et al. 2016. PLoS One. 11: 0152485. PubMed
  17. Mangum L, et al. 2016. Toxicol Sci. 150: 169 - 177. PubMed
  18. Chng S, et al. 2016. Sci Rep. 6: 23820. PubMed
  19. Ahmed M, et al. 2016. Development. 143: 1663 - 1673. PubMed
  20. Eastman A, et al. 2016. J Immunol . 194: 5999-6010. PubMed
  21. Yuan Z, et al. 2016. J Virol. 90: 7728 - 7739. PubMed
  22. Wong J, et al. 2016. Sci Signal. 9: ra103. PubMed
  23. Chiche A, et al. 2016. Oncogene. 10.1038/onc.2016.396. PubMed
  24. Mueller A, et al. 2016. Nature. 540:276-279. PubMed
  25. Vaas M, et al. 2016. J Cereb Blood Flow Metab. 10.1177/0271678X16676825. PubMed
  26. Mizuno T, et al. 2017. Sci Rep. 7:42714. PubMed
  27. Paiva-Oliveira E, et al. 2017. Histochem Cell Biol. 10.1007/s00418-017-1556-6. PubMed
  28. An J,et al. 2017. Sci Rep.. 10.1038/s41598-017-13629-0. PubMed
  29. Feng J,et al. 2017. Nat Commun. . 10.1038/s41467-017-01056-8. PubMed
  30. Pal B, et al. 2017. Nat Commun.. 10.1038/s41467-017-01560-x. PubMed
  31. Zheng B, et al. 2018. J Hematol Oncol. 11:16. PubMed
  32. Su M, et al. 2018. Immunol Cell Biol. 9:2448. PubMed
  33. Kabir AU, et al. 2018. JCI Insight. 3. PubMed
  34. Gavin KM, et al. 2018. Front Endocrinol (Lausanne). 9:276. PubMed
  35. Shavit Stein E, et al. 2018. Neural Plast. 2018:7692182. PubMed
  36. Wunderlich CM, et al. 2018. Nat Commun. 9:1646. PubMed
  37. Wang L, et al. 2018. Bone Res. 6:22. PubMed
  38. Yuan Z, et al. 2018. Emerg Microbes Infect. 7:59. PubMed
  39. Echevarría-Vargas IM, et al. 2018. EMBO Mol Med. 10:e8446. PubMed
  40. Porrello A, et al. 2018. Nat Commun. 9:1988. PubMed
  41. Cha JH 2018. Molecular cell. 71:606. PubMed
  42. Liu L 2018. Cell stem cell. 23:544. PubMed
  43. Sarshad AA 2018. Molecular cell. 71:1040. PubMed
  44. Shi R, et al. 2019. J Cancer. 10:367. PubMed
  45. Qiao S, et al. 2019. J Neuroinflammation. 16:4. PubMed
  46. Chiche A, et al. 2019. Breast Cancer Res. 21:13. PubMed
  47. Gao Q, et al. 2019. J Exp Med. 216:688. PubMed
  48. Xiao X, et al. 2019. Cell Discov. 5:2. PubMed
  49. Peltzer N, et al. 2018. Nature. 557:112. PubMed
  50. Piotrowski AF, et al. 2018. Oncoimmunology. 7:e1445951. PubMed
  51. Subramanian K, et al. 2019. Nat Microbiol. 4:62. PubMed
  52. Cao Y, et al. 2019. Nat Commun. 10:1280. PubMed
  53. Wosczyna MN, et al. 2019. Cell Rep. 27:2029. PubMed
Publication Library
RRID
AB_312976 (BioLegend Cat. No. 103111)
AB_312977 (BioLegend Cat. No. 103112)

Antigen Details

Structure
Protein tyrosine phosphatase (PTP) family, 180-240 kD
Distribution

All hematopoietic cells except mature erythrocytes and platelets

Function
Phosphatase, T and B cell activation
Ligand/Receptor
Galectin-1, CD2, CD3, CD4, TCR, CD22, Thy-1
Cell Type
Mesenchymal Stem Cells, B cells, Dendritic cells, Tregs
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroscience, Neuroscience Cell Markers, Stem Cells, Inhibitory Molecules
Molecular Family
CD Molecules
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Trowbridge IS, et al. 1993. Annu. Rev. Immunol. 12:85.
3. Kishihara K, et al. 1993. Cell 74:143.
4. Pulido R, et al. 1988. J. Immunol. 140:3851.

Gene ID
19264 View all products for this Gene ID
UniProt
View information about CD45 on UniProt.org

Related FAQs

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Go To Top Version: 2    Revision Date: 05/14/2013

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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