APC anti-mouse CD3ε Antibody

Pricing & Availability
Clone
145-2C11 (See other available formats)
Other Names
CD3ε, T3, CD3
Isotype
Armenian Hamster IgG
Ave. Rating
4 reviews
Product Citations
publications
145-2C11_APC_021606
C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
  • 145-2C11_APC_021606
    C57BL/6 mouse splenocytes were stained with CD3e (clone 145-2C11) APC (filled histogram) or Armenian hamster IgG APC isotype control (open histogram).
See APC spectral data
Cat # Size Price Quantity Avail. Save
100311 25 µg $30
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100312 100 µg $95
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Description

CD3ε is a 20 kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3ε forms a TCR complex by associating with the CD3δ, γ and ζ chains, as well as the TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The CD3ε antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Clone 145-2C11 is useful for in vitro blocking of target-specific CTL-mediated cell lysis1, as well as T cell activation assays, inducing proliferation and cytokine production1,2,7,12,16. It also induces apoptosis in immature thymocytes32,  and in vivo T cell depletion8-10. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation1, immunohistochemical staining14,15 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, Western blotting4, complement-mediated cytotoxicity6, in vitro and in vivo stimulation of T cells1,2,7,12,16, immunofluorescent staining5, and in vivo T cell depletion8-10. The 145-2C11 antibody has been reported to block the binding of 17A2 antibody to CD3 epsilon-specific T cells11. Clone 145-2C11 is not recommended for formalin-fixed paraffin embedded sections. The LEAF™ purified antibody (Endotoxin <0.1 EU/μg, Azide-Free, 0.2 μm filtered) is recommended for functional assays (Cat. No. 100314). For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 100340) with a lower endotoxin limit than standard LEAF™ purified antibodies (Endotoxin <0.01 EU/µg).

Application References

(PubMed link indicates BioLegend citation)
  1. Leo O, et al. 1987. P. Natl. Acad. Sci. USA 84:1374. (IP, Activ, Block)
  2. Kruisbeek AM, et al. 1991. In Current Protocols in Immunology. 3.12.1. (Activ)
  3. Duke RC, et al. 1995. Current Protocols in Immunology. 3.17.1.
  4. Salvadori S, et al. 1994. J. Immunol. 153:5176. (WB)
  5. Payer E, et al. 1991. J. Immunol. 146:2536. (IF)
  6. Jacobs H, et al. 1994. Eur. J. Immunol. 24:934. (CMCD)
  7. Vossen ACTM, et al. 1995. Eur. J. Immunol. 25:1492. (Activ)
  8. Henrickson M, et al. 1995. Transplantation 60:828. (Deplete)
  9. Kinnaert P, et al. 1996. Transpl. Int. 9:386. (Deplete)
  10. Han WR, et al. 1999. Transpl. Immunol. 7:207. (Deplete)
  11. Miescher GC, et al. 1989. Immunol. Lett. 23:113. (Block)
  12. Terrazas LI, et al. 2005. Intl. J. Parasitology. 35:1349. (Activ)
  13. Ko SY, et al. 2005. J. Immunol. 175:3309.
  14. Podd BS, et al. 2006. J. Immunol. 176:6532. (IHC-F)
  15. Tilley SL, et al. 2007. J. Immunol. 178:3208. (IHC-F)
  16. Wang W, et al. 2007. J. Immunol. 178:4885. (Activ)
  17. Xiao S, et al. 2007. J. Exp. Med. 204:1691.
  18. Chappaz S, et al. 2007. Blood doi:10.1182/blood-2007-02-074245. (FC) PubMed.
  19. Curtsinger JM, et al.2005. J. Immunol. 175:4392. PubMed
  20. Guo Y, et al. 2008. Blood 112:480. PubMed
  21. Kenna TJ, et al. 2008. Blood 111:2091.
  22. Perchonock CE, et al. 2007. J. Immunol. 179:1768. PubMed
  23. Perchonock GE, et al. 2006. Mol. Cell. Biol. 26:6005. PubMed
  24. Kanaya T, et al. 2008. Am. J. Physiol. Gastrointest. Liver Physiol. 295:G273. PubMed
  25. de Koning BA, et al. 2006. Int. Immunol. 18:941. PubMed
  26. Schulteis RD, et al. 2008. Blood 295:G273. PubMed
  27. Qi Q, et al. 2009. Blood 114:564. PubMed
  28. Helmersson S, et al. 2013. Am J Pathol. 9440:123. Pubmed
  29. Wu S, et al. 2014. Clin Vaccine Immunol. 21:156. PubMed
  30. Yan J, et al. 2014. Vaccine. 32:2833. PubMed
  31. Guiterrez DA, et al. 2014. Diaebetes. 63:3827. PubMed
  32. Shi YF, et al. 1991. J Immunol. 146:3340. (Apop)
Product Citations
  1. Huang J, et al. 2014. J Immunol. 192:1972. PubMed
  2. Okamoto M, et al. 2014. J Immunol. 192:2770. PubMed
  3. Zhu E, et al. 2014. J Immunol. 192:5599. PubMed
  4. Aebischer D, et al. 2014. PLoS One. 9:99297. PubMed
  5. Bergquist M, et al. 2014. J Infect. 69:113. PubMed
  6. Pennock N, et al. 2014. Proc Natl Acad Sci U S A. 111:16472. PubMed
  7. Nakamura M, et al. 2015. J Immunol. 194:243. PubMed
  8. Burrack K, et al. 2015. J Immunol. 194:678. PubMed
  9. Wan W, et al. 2015. Cardiovasc Res. 106:478. PubMed
  10. Luck H, et al. 2015. Cell Metab. 21 527 . PubMed
  11. XS R, et al. 2015. Diabetes. 64 90. PubMed
  12. Sato K, et al. 2015. PLoS One. 10: 0138291. PubMed
  13. Siggs O, et al. 2015. Proc Natl Acad Sci U S A. 112: E5706 - E5714. PubMed
  14. Burrack K, et al. 2015. PLoS Pathog. 11: e1005191. PubMed
  15. Ito C, et al. 2015. PLoS One. 10: e0140808. PubMed
  16. Xu G, et al. 2015. Free Radic Biol Med. 87: 15-25. PubMed
  17. Holvoet B, et al. 2015. Stem Cell Reports. 5: 1183-1195. PubMed
  18. Rovira-Clavé X, et al. 2016. J Leukoc Biol. 99: 143 - 152. PubMed
  19. Leiva M, et al. 2016. Nat Commun. 7:10222. PubMed
  20. Pelly V, et al. 2016. Mucosal Immunol. 10.1038/mi.2016.4. PubMed
  21. Uto T, et al. 2016. Nat Commun. 7: 11273. PubMed
  22. Cruz F, et al. 2016. Stem Cells Trans Med. 5: 488-499. PubMed
  23. Campisi L, et al. 2016. Nat Immunol. 10.1038/ni.3512. PubMed
  24. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  25. Ku M, et al. 2016. Mol Cancer Ther. 15: 1975 - 1987. PubMed
  26. Wang Y, et al. 2016. Sci Rep. 6:31881. PubMed
  27. Roufaiel M, et al. 2016. Nat Immunol. 10.1038/ni.3564. PubMed
  28. Miura N, et al. 2017. Mol Ther. 10.1016/j.ymthe.2017.01.020. PubMed
  29. Harvey R, et al. 2017. Endocrinology. 10.1210/en.2016-1832. PubMed
  30. Glasner A,et al. 2017. Sci Rep.. 10.1038/s41598-017-12998-w. PubMed
  31. Wang J, et al. 2018. Front Immunol. 9:371. PubMed
  32. Liu S, et al. 2018. Front Immunol. 29:208. PubMed
  33. Xia P 2018. Immunity. 48:688. PubMed
  34. Gubin MM 2018. Cell. 175:1014. PubMed
  35. Metidji A 2018. Immunity. 49:353. PubMed
  36. Celik H 2018. Cancer cell. 34:741. PubMed
  37. Kang YH, et al. 2019. Nat Commun. 10:912. PubMed
  38. Tong Y, et al. 2018. EBioMedicine. 39:132. PubMed
  39. Agelidis A, et al. 2019. Front Immunol. 10:500. PubMed
  40. Ajina R, et al. 2019. Oncoimmunology. 8:e1577127. PubMed
Publication Library
RRID
AB_312676 (BioLegend Cat. No. 100311)
AB_312677 (BioLegend Cat. No. 100312)

Antigen Details

Structure
Ig superfamily, forms CD3/TCR complex with CD3δ, γ and ζ subunits and TCR (α/β and γ/δ), 20 kD
Distribution

Thymocytes (differentiation dependent), mature T cells, NK-T cells

Function
TCR signal transduction, T cell activation, antigen recognition
Ligand/Receptor
Peptide antigen/MHC-complex
Cell Type
NKT cells, T cells, Thymocytes, Tregs
Biology Area
Immunology
Molecular Family
CD Molecules, TCRs
Antigen References

1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475.
3. Weiss A, et al. 1994. Cell 76:263.

Gene ID
12501 View all products for this Gene ID
UniProt
View information about CD3e on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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This data display is provided for general comparisons between formats.
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