APC anti-mouse CD326 (Ep-CAM) Antibody

Pricing & Availability
Clone
G8.8 (See other available formats)
Other Names
CD326, EGP40, MIC18, TROP1, KSA
Isotype
Rat IgG2a, κ
Ave. Rating
2 reviews
Product Citations
publications
G8dot8_APC_081908.jpg
TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
  • G8dot8_APC_081908.jpg
    TE-71 (mouse thymic epithelial stromal cell line) stained with G8.8 APC
See APC spectral data
Cat # Size Price Quantity Avail. Save
118213 25 µg $85
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118214 100 µg $250
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Description

EpCAM (CD326) mediates calcium-independent homophilic cell to cell adhesion. It may also function as a growth factor receptor. It is thought to be involved in maintaining cells in position during proliferation. Expression of EpCAM seems to correlate inversely with the level of E-cadherin (CD324). EpCAM is considered important in tumor biology.

Product Details
Technical data sheet

Product Details

Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
TE-71 thymic epithelial cell line
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions. The solution is free of unconjugated APC and unconjugated antibody.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested

Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications for clone G8.8 (for the relevant formats) include: immunohistochemistry of frozen sections: acetone fixed1, with or without OCT embedding2,4.

Application References

(PubMed link indicates BioLegend citation)
  1. Farr A, et al. 1991. J. Histochem. Cytochem. 39:645. (FC, IHC)
  2. Dooley J, et al. 2005. J. Immunol. 175:4331. (FC, IHC)
  3. Hinterberger M, et. al. 2010. Nat. Immunol. 11:512. (FC) PubMed
  4. Gracz AD, et al. 2010. Am J. Physiol Gastrointest Liver Physiol. 298:590. (IHC) PubMed
  5. Nudel I, et al. 2011. J. Immunol. 186:891. PubMed
  6. Morimoto H, et al. 2012. Biol Reprod. 86:148. PubMed
  7. Ishii K, et al. 2012. Development. 139:1734. PubMed
  8. Takehashi M, et al. 2012. Biol Reprod. 86:178. PubMed
  9. Murakami R, et al. 2013. PLoS One. 8:73270. PubMed
  10. Taguchi K, et al. 2014. Mol Cell Biol. 34:900. PubMed
  11. Hirokawa Y, et al. 2014. Am J Physiol Gastrointerest Liver Physiol. 306:547. PubMed
  12. Ding X, et al. 2015. Cancer Res. 75:330. PubMed
Product Citations
  1. Shultz L, et al. 2010. Proc Natl Acad Sci U S A. 107:13022. PubMed
  2. Casey O, et al. 2012. PLoS One. 7:e41668. PubMed
  3. King J, et al. 2012. Am J Physiol Gastrointest Liver Physiol. 303:443. PubMed
  4. Tanimizu N, et al. 2014. J Biol Chem. 289:7859. PubMed
  5. Serra-Hassoun M, et al. 2014. J Immunol. 193:1504. PubMed
  6. Wang Y, et al. 2014. PLoS One. 9:113127. PubMed
  7. Barone F, et al. 2015. Proc Natl Acad Sci U S A. 112: 11024-11029. PubMed
  8. Elkon R, et al. 2015. Nat Commun. 6: 8549. PubMed
  9. Buerger S, et al. 2015. J Immunol. 195: 4106 - 4116. PubMed
  10. Malhotra D, et al. 2016. Nat Immunol. 17:187-195. PubMed
  11. Baptista M, et al. 2016. Nat Commun. 7:12175. PubMed
  12. Tripathi D, et al. 2016. Nat Commun. 7:13896. PubMed
  13. Chua CW, et al. 2018. Elife. 7. PubMed
  14. Smith NR, et al. 2018. Cell Mol Gastroenterol Hepatol. 6:79. PubMed
  15. Tippimanchai DD, et al. 2018. Oncoimmunology. 7:e1438105. PubMed
  16. Guo XJ 2018. Immunity. 49:531. PubMed
  17. Malik A 2018. Immunity. 49:515. PubMed
  18. Cohen I 2018. Cell stem cell. 22:726. PubMed
  19. Renz BW 2018. Cancer cell. 33:75. PubMed
  20. Finn J, et al. 2019. Cell Rep. 26:2942. PubMed
  21. Komban RJ, et al. 2019. Nat Commun. 10:2423. PubMed
Publication Library
RRID
AB_1134105 (BioLegend Cat. No. 118213)
AB_1134102 (BioLegend Cat. No. 118214)

Antigen Details

Structure
40 kD single-pass type 1 glycoprotein. 293 amino acids, with a 21 aa signal peptide, a 246 aa extracellular domain, a 21 aa transmembrane domain, and a 26 aa cytoplasmic domain. The extracellular domain contains two epidermal growth factor-like repeats.
Distribution

Expressed on majority of epithelial cell membranes with the exception of adult squamous cells of the skin and a few specific epithelial cell types.

Function
Mediates calcium-independent homophilic cell-cell adhesion.
Interaction
CD326 displays hemophilic binding.
Ligand/Receptor
CD305 (LAIR-1), CD306 (LAIR-2), and Ep-CAM.
Cell Type
Embryonic Stem Cells, Epithelial cells
Biology Area
Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Borkowski TA, et al. 1996. Eur. J. Immunol. 26:110.
2. Bergsagel PL, et al. 1992. J. Immunol. 148:590.

Gene ID
17075 View all products for this Gene ID
UniProt
View information about CD326 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 11/30/2012

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