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APC anti-mouse CD317 (BST2, PDCA-1) Antibody

Pricing & Availability
Clone
927 (See other available formats)
Regulatory Status
RUO
Other Names
BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, PDCA-1
Isotype
Rat IgG2b, κ
Ave. Rating
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Product Citations
publications
A_927_APC_011310
C57BL/6 splenocytes stained with 927 APC and CD45R/B220 (RA3-6B2) PE
  • A_927_APC_011310
    C57BL/6 splenocytes stained with 927 APC and CD45R/B220 (RA3-6B2) PE
  • B_927_APC_011310
    C57BL/6 splenocytes stained with rat IgG2b APC isotype control and CD45R/B220 (RA3-6B2) PE
Compare all formats See APC spectral data
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127015 25 µg $107
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127016 100 µg $350
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Description

CD317, known as BST2, tetherin, HM1.2 antigen, bone marrow stromal antigen 2, or PDCA-1, is type II transmembrane glycoprotein with a molecular mass of 29-33 kD. It is predominantly expressed on Type I IFN-producing cells (IPCs) in naïve mice, but is up-regulated on most cell types following stimulation with type I IFNs and IFN-gamma. It is highly expressed on terminally differentiated normal plasmacytoid dendritic cells and some tumor cells, such as multiple myeloma, renal cell carcinoma, and melanoma cells. BST2 is a recently identified, IFN-induced cellular response factor that blocks release of HIV-1 and other retroviruses from infected cells. BST2 has been found to be the natural ligand of ILT7 in human model.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse plasmacytoid dendritic cells (DCs)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with APC under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.06 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Red Laser (633 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy, functional assay2, and depletion3,4. The Ultra-LEAF™ purified antibody (Endotoxin <0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Blasius AL, et al. 2006. J. Immunol. 177:3260.
  2. Schliemann C, et al. 2010. Blood 115:736. (FA, IF)
  3. Rajagopal D, et al. 2010. Blood 115:1949. (Depletion)
  4. Moniz RJ, et al. 2010. FEMS Immunol. Med. Microbiol. 58:397. (Depletion)
  5. Chen YL, et al. 2013. J Exp Med. 210:2515. PubMed
Product Citations
  1. Wagner C, et al. 2020. Cell Reports. 31(1):107479. PubMed
  2. Kitagawa S, et al. 2022. NPJ Vaccines. 7:115. PubMed
  3. Rødahl I, et al. 2021. STAR Protoc. 2:100842. PubMed
  4. Findlay EG, et al. 2019. Oncoimmunology. 8:1608106. PubMed
  5. Go DM, et al. 2021. Cell Mol Gastroenterol Hepatol. 12:715. PubMed
  6. Billi AC, et al. 2019. JCI Insight. 4:8. PubMed
  7. Han Y, et al. 2015. J Immunol. 195: 4126 - 4135. PubMed
  8. Grzelak A, et al. 2018. Int J Mol Sci. 19:. PubMed
  9. Mulas F, et al. 2020. Cell Mol Immunol. . PubMed
  10. Sauma D, et al. 2017. Immunol Res. 10.1007/s12026-017-8936-9. PubMed
  11. Martínez‐López M et al. 2019. Immunity. 50(2):446-461 . PubMed
  12. Yang P et al. 2019. Cell Syst. 8(5):427-445 . PubMed
  13. Bi E, et al. 2018. J Clin Invest. 128:4821. PubMed
  14. Hirata SI, et al. 2020. Allergy. 75:1939. PubMed
  15. Matsuda T, et al. 2022. iScience. 25:105324. PubMed
  16. Younes AI, et al. 2021. Transl Oncol. 14:100983. PubMed
  17. Walker MT, et al. 2021. Front. Allergy. 2:. PubMed
  18. Conrad C, et al. 2018. Nat Commun. 9:25. PubMed
  19. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
RRID
AB_1967101 (BioLegend Cat. No. 127015)
AB_1967127 (BioLegend Cat. No. 127016)

Antigen Details

Structure
Type II transmembrane glycoprotein with a molecular mass of 29-33 kD.
Distribution

Expressed on type I IFN-producing cells, plasmacytoid dendritic cells, and neoplastic B cells, such as multiple myeloma.

Function
Recently identified antiviral protein that blocks the release of nascent retrovirus or other particles from infected cells.
Cell Type
Dendritic cells
Biology Area
Costimulatory Molecules, Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Douglas JL. et al. 2009. J Virol. 83(16):7931
2. Cao W et al. 2009. J. Exp. Med. 206(7):1603
3. Neil SJ. et al. 2008. Nature 451:425

Gene ID
69550 View all products for this Gene ID
UniProt
View information about CD317 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 03/04/2013

For research use only. Not for diagnostic use. Not for resale. BioLegend will not be held responsible for patent infringement or other violations that may occur with the use of our products.

 

*These products may be covered by one or more Limited Use Label Licenses (see the BioLegend Catalog or our website, www.biolegend.com/ordering#license). BioLegend products may not be transferred to third parties, resold, modified for resale, or used to manufacture commercial products, reverse engineer functionally similar materials, or to provide a service to third parties without written approval of BioLegend. By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses. Unless otherwise indicated, these products are for research use only and are not intended for human or animal diagnostic, therapeutic or commercial use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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